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pEGFP-N1_MeCP2(deltaN)

Plasmid

#110189

Purpose

Expression of mouse MeCP2 (deltaN) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 delta N (mouse cDNA) _EGFP (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

delta N terminus

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.TRC1.shmNsd1.2, puro

Plasmid

#114447

Purpose

Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

Depositor

Adrian Bracken

Article

Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

Insert

shNsd1.2 (Nsd1 Mouse)

Use

Lentiviral and RNAi

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCR8-CHD5-FRAGMENT

Plasmid

#68879

Purpose

GW entry vector for CHD5

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Gateway Cloning

Mutation

331-672aa

Promoter

none

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pMIHP-16167 AU1.Flag.Ollas

Plasmid

#241479

Purpose

AU1.Flag.Ollas Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, Flag, and Ollas

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16170 AU1.FLAG.V5

Plasmid

#241480

Purpose

AU1.FLAG.V5 Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, FLAG, and V5

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16173 AU1.HA.Ollas

Plasmid

#241481

Purpose

AU1.HA.Ollas Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, HA, and Ollas

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16186 AU1.Ollas.S

Plasmid

#241484

Purpose

AU1.Ollas.S Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, Ollas, and S

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16188 AU1.OLLAS.V5

Plasmid

#241485

Purpose

AU1.OLLAS.V5 Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, OLLAS, and V5

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16175 AU1.HA.VSVG

Plasmid

#241497

Purpose

AU1.HA.VSVG Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, HA, and VSVG

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16187 AU1.OLLAS.VSVG

Plasmid

#241498

Purpose

AU1.OLLAS.VSVG Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, OLLAS, and VSVG

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16189 AU1.S.VSVG

Plasmid

#241499

Purpose

AU1.S.VSVG Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, S, and VSVG

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16191 AU1.VSVG.V5

Plasmid

#241500

Purpose

AU1.VSVG.V5 Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Article

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, V5, and VSVG

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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shRNA_circSMAD2_2

Plasmid

#215222

Purpose

Supression of shcircSMAD2(2,7)_2 expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

circSMAD2 shRNA 2 (SMAD2 Human)

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-DRD1-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194361

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-DRD1-V2R-NTEV-TCS-GV-2xHA (DRD1 Synthetic, Human)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-CHD5-WT-FRAG

Plasmid

#68873

Purpose

IPTG inducible bacterial expression of CHD5 fragment

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Tags

GST

Expression

Bacterial

Mutation

331-672aa

Promoter

tac

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
MultiMate-PE3 HEK3

Plasmid

#206273

Purpose

Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE3 HEK3 prime editing.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP

Use

CRISPR; Recombinant baculovirus production

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry GRK2 ct in pcDNA3.1

Plasmid

#64212

Purpose

mCherry fused between Cry2 and GRK2ct to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK2 ct (GRK2 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV and cmv

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-N1_MeCP2(deltaNIC)

Plasmid

#110191

Purpose

Expression of mouse MeCP2 (deltaNIC) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 delta NIC (mouse cDNA) - EGFP (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

delta N and C termini. Intervening region replace…

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {hCAR}off-{ChETA}on-WPRE

Plasmid

#111388

Purpose

AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

hCAR (CXADR Human)
ChETA

Use

AAV

Tags

HA and Myc

Expression

Mammalian

Promoter

hSynapsin

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry GRK3 ct in pcDNA3.1

Plasmid

#64213

Purpose

mCherry fused between Cry2 and GRK3 ct to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK3 ct (GRK3 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only