-
Plasmid#130938PurposeEngineered sgRNA-LEB1 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEB1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pFTK090
Plasmid#171362PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsert3'flanking region - penicillin gene cluster P. rubens
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK089
Plasmid#171361PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsert5'flanking region - penicillin gene cluster P. rubens
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTSA-38
Plasmid#17328DepositorInsertstreptavidin
UseTagsExpressionBacterialMutationTruncated to include only amino acids 16-133 of m…PromoterAvailable sinceFeb. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
-
Gli3ZF in AINGpCAGGS
Plasmid#121972PurposeGli3 zinc fingersDepositorInsertGli3ZF (GLI3 Human)
UseChick electroporationTags5xmycExpressionMammalianMutationN terminus and C terminus deletion only nt 1411-1…PromoterAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDH5
Plasmid#83775PurposeUseful for tagging yeast proteins with C-terminal YFP tag; his selectionDepositorInsertYFP
UseTagsYFPExpressionYeastMutationS65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…PromoterAvailable sinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
DPY-pNCS
Plasmid#17365DepositorInsertMMLV proviral DNA
UseTagsExpressionMutationdeletion that removes 5 amino acids (DPPPY) from …PromoterAvailable sinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLY103
Plasmid#130937PurposeEngineered sgRNA-LEA1 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEA1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAA
Plasmid#78487Purpose2135nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tailDepositorInsertCAN1-LUC
UseTagsExpressionBacterialMutationCAN1 contains no stop codonPromoterAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-RPL13A-FKBPx4
Plasmid#104474PurposePlasmid for pop-in/pop-out replacement of RPL13A with an FKBPx4-tagged version.DepositorInsertRPL13Ap (RPL13A Budding Yeast)
UseTagsFKBPx4-HAExpressionYeastMutationincludes the last 354 bp of RPL13A onlyPromoterAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNP154
Plasmid#106360PurposeA vector backbone, including mKate, to be used to make constructs to be inserted as a single copy at a site on Chr II.DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pLSLZ.D
Plasmid#51496PurposepLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmidDepositorInsertsZif268:FokI
us:NPTII repair template
UsePlant t-dna plasmidTagsSV40 NLSExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
POSV801-SP44-SnaABCDEOT1T2
Plasmid#225055PurposeHeterologous expression in Streptomyces for threopeptin biosynthetic gene clusterDepositorInsertSnaA
UseTagsExpressionBacterialMutationWTPromoterSP44Available sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC3-N-GFP-NES
Plasmid#222606PurposeExpression of MAC3-tagged GFP-NES (N-terminal)DepositorInsertEGFP and 3' nuclear exclusion sequence (NES)
UseTagsMAC3ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceAug. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC3-C-GFP-NES
Plasmid#222603PurposeExpression of MAC3-tagged GFP-NES (C-terminal)DepositorInsertEGFP and 5' nuclear exclusion sequence (NES)
UseTagsMAC3ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceAug. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-RPL3-72~197-mEGFP(A206K)-TwStrep
Plasmid#208625Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa72~197, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 72~197 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 72~197, other residues not includedPromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ARL16-His6
Plasmid#216484PurposeUsed for exogenous expression of human ARL16, short form, in mammalian cells. The encoded protein contains a C-terminal, glycine rich linker and His6 tagDepositorInsertADP-ribosylation factor like 16 (ARL16 Human)
UseTagsincludes GSGSGSHHHHHH (His6) tag at C-terminusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only