Addgene

Showing: 1621 - 1640 of 32215 results

Results per page:

Sort results by:

pdest METTL1-WT Neo

Plasmid

#223058

Purpose

METTL1 gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

METTL1 (METTL1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCmini-iCAP-AAV1.X1

Plasmid

#209780

Purpose

non-standard AAV2 rep-AAV1-X1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

Insert

Synthetic construct isolate AAV1-X1 VP1 gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

p41

Available since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)

Plasmid

#209077

Purpose

Ngn2 mediated cortical neuron induction, dox induction is included

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

NGN2 (Neurog2 Mouse)
SNAPtag

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE4G

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GST-GGA3-VHS-GAT

Plasmid

#173719

Purpose

VHS-GAT domain in GGA3 can bind to an active form of ARF1 protein. Using this construct, you can assess the activity of ARF1 protein.

Depositor

Juan Bonifacino

Article

Dell'Angelica et al J Cell Biol. 2000 Apr 3;149(1):81-94. do

Insert

GGA3 (GGA3, GGA2, GGA1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIPZ-p21-HA(RxL)

Plasmid

#215111

Purpose

Expresses p21-HA(RxL) in mammalian cells from an inducible lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

p21(RxL) denotes mutations: R19A, R20A, L21A, F22…

Promoter

CMV (Tet inducible)

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRIPZ-p21-HA(dPCNA)

Plasmid

#215112

Purpose

Expresses p21-HA(dPCNA) in mammalian cells from an inducible lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

p21(dPCNA) denotes mutations M147A, D149A, F150A.

Promoter

CMV (Tet inducible)

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT1219

Plasmid

#228301

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable expression of SARS-CoV-2 RBD omicron variant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

MFalpha(2xAdv)-SARS CoV-2 receptor binding domain (RBD)-His (S Synthetic, Severe acute respiratory syndrome-related coronavirus 2)

Use

Synthetic Biology

Tags

GGG linker followed by His6 and MF_ secretion sig…

Expression

Yeast

Mutation

G339D, S371L, S373P, S375F, K417N, N440K, G446S, …

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pdest N-FLAG METTL1-WT puro

Plasmid

#223055

Purpose

METTL1-WT gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

METTL1-WT (METTL1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
rag2:LRRC10_CE_pISceI

Plasmid

#223042

Purpose

Express LRRC10 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

LRRC10 (LRRC10 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
rag2:BEST3_CE_pISceI

Plasmid

#223043

Purpose

Express BEST3 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

BEST3 (BEST3 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
rag2:CNOT_CE_pISceI

Plasmid

#223046

Purpose

Express CNOT2 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

CNOT2 (CNOT2 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
rag2:MARCH9_CE_pISceI

Plasmid

#223027

Purpose

Express MARCH--9 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

MARCH--9 (MARCH9 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
rag2:METTL1_CE_pISceI

Plasmid

#223029

Purpose

Express METTL1 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

METTL1 (METTL1 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
rag2:AVIL_CE_pISceI

Plasmid

#223032

Purpose

Express AVIL gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

AVIL (AVIL Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
rag2:MDM2_CE_pISceI

Plasmid

#223035

Purpose

Express MDM2 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

MDM2 (MDM2 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
rag2:CPM_CE_pISceI

Plasmid

#223036

Purpose

Express CPM gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

CPM (CPM Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
rag2:AGAP2_CE_pISceI

Plasmid

#223024

Purpose

Express AGAP2 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

AGAP2 (AGAP2 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
rag2:CDK4_CE_pISceI

Plasmid

#223026

Purpose

Express CDK4 gene in mesenchymal lineage of Zebrafish.

Depositor

Alejandro Gutierrez

Article

Raja H. et al Molecular Cell, 2025, ISSN 1097-2765

Insert

CDK4 (CDK4 Human)

Use

Express the insert(gene) in mesenchymal lineage o…

Tags

Expression

Mutation

Promoter

Rag2

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PGL4.11-COL1A1mTEAD

Plasmid

#230916

Purpose

Comparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.

Depositor

Avi Rosenberg

Article

Yang et al Lab Invest. 2024 May;104(5):100336. doi:

Insert

Human COL1A1 promoter with mutant TEAD elements (COL1A1 Human)

Use

Luciferase

Tags

Luciferase- luc2p

Expression

Bacterial and Mammalian

Mutation

mutant potential TEAD bind elements AGGAAT to CTG…

Promoter

COL1A1

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-TET-MKOS

Plasmid

#20959

Purpose

piggyBac vector with tet inducible cMyc, KLF4, Oct4, Sox2 for creation of induced pluripotent stem cells

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

cMyc, KLF4, Oct4, Sox2 (Myc, Klf4, Pou5f1, Sox2 Mouse)

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge