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We narrowed to 7,608 results for: Pol

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Showing: 1621 - 1640 of 7608 results

  1. pET21 MBPx-SpyCatcher

    Plasmid
    #72327
    Purpose
    Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.
    Depositor
    Mark Howarth
    Article
    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p
    Insert
    pET21 MBPx-SpyCatcher
    Tags
    N-terminal His6 tag
    Expression
    Bacterial
    Mutation
    MBP contains A312V, I317V mutations and 172, 173,…
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72327-plasmid-map-sequence-id-434061

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  2. Fireworks GFP[PTC-] (AVA2598)

    Plasmid
    #85445
    Purpose
    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.
    Depositor
    Joan Steitz
    Article
    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:
    Insert
    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Expression
    Mammalian
    Available Since
    Feb. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    85445-plasmid-map-sequence-id-171491

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  7. pET-21b(+)-Is-PETase-W185A

    Plasmid
    #112204
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W185A
    Promoter
    N/A
    Available Since
    July 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    112204-plasmid-map-sequence-id-215853

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  15. pBRα

    Plasmid
    #53731
    Purpose
    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    αNTD
    Expression
    Bacterial
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available Since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    53731-plasmid-map-sequence-id-83909

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  19. pSL1180-HR-PUbECFP

    Plasmid
    #47917
    Purpose
    Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
    Depositor
    Leslie Vosshall
    Insert
    ECFP
    Tags
    NLS
    Expression
    Insect
    Promoter
    Aedes aegypti polyubiquitin (PUb)
    Available Since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    47917-plasmid-map-sequence-id-68604

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Showing: 1621 - 1640 of 7608 results