We narrowed to 159,258 results for: addgene

1. pAG415Gal_DBDGFP

   Plasmid

   #232999

   Purpose

   Galactose iduced expression of Gcn4 DBDGFP in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   DBD
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHR_5xUAS-ETV2-P2A-BFP-WPRE_PGK-HygR

   Plasmid

   #216664

   Purpose

   Expressed Gal4-inducible ETV2 and BFP, with hygromycin resistance

   Depositor

   Leonardo Morsut

   Inserts

   ETV2 (Etv2 Mouse)
   BFP
   HygromycinR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSF503

   Plasmid

   #222587

   Purpose

   BB3 gsdA tet-on overexpression cassette with homologous flanks for integration at pyrG locus

   Depositor

   Matthias Steiger

   Article

   Matthias et al PLOS One

   Insert

   gsdA (gsdA )
   

   Use

   A. niger

   Tags

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert,…

   Promoter

   tet-on

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSF8

   Plasmid

   #222592

   Purpose

   BB2 gsdA tet-on overexpression cassette

   Depositor

   Matthias Steiger

   Article

   Matthias et al PLOS One

   Insert

   gsdA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert,…

   Promoter

   tet-on

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTYC10_pPromALB_STARRseq

   Plasmid

   #220289

   Purpose

   STARR-seq plasmid driven by minimal mouse Alb promoter; used to measure enhancer activity of a library of DNA sequences on interest. Useful for STARR-seq analysis in liver.

   Depositor

   David Waxman

   Article

   Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCJ1022

   Plasmid

   #228762

   Purpose

   Plasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.

   Depositor

   Bradley Yoder

   Insert

   Pkd2 gRNAs (Pkd2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 8V04

   Plasmid

   #220879

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Baculovirus expresssion

   Tags

   EFVEHHHHHHHH and Honeybee melittin signal

   Expression

   Mutation

   S251D/R252D/Q253D/S254D/R255K

   Promoter

   Polyhedrin

   Available since

   Sept. 30, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. lentiGuide-dsRed

   Plasmid

   #217006

   Purpose

   lentiviral plasmid for gRNA cloning with selectable dsRed expression

   Depositor

   Deepta Bhattacharya

   Article

   D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. MAC3-N-GFP

   Plasmid

   #222602

   Purpose

   Expression of MAC3-tagged GFP (N-terminal)

   Depositor

   Markku Varjosalo

   Article

   Liu et al Methods Mol Biol. 2023;2690:281-297. doi

   Insert

   EGFP
   

   Use

   Tags

   MAC3

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. MAC3-C-GFP

    Plasmid

    #222601

    Purpose

    Expression of MAC3-tagged GFP (C-terminal)

    Depositor

    Markku Varjosalo

    Article

    Liu et al Methods Mol Biol. 2023;2690:281-297. doi

    Insert

    EGFP
    

    Use

    Tags

    MAC3

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pHR-IDR4-mCh-Cry2WT-NLS

    Plasmid

    #221920

    Purpose

    Expression of CBP IDR4 fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP IDR4 (aa 662-787) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the IDR4 region (aa 662-787); insert…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHR-CFID-mCh-Cry2WT-NLS

    Plasmid

    #221923

    Purpose

    Expression of CBP CFID fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP CFID (aa 1892-2441) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the CBP-FUS interaction domain (CFID…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR-IDR3-mCh-Cry2WT-NLS

    Plasmid

    #221919

    Purpose

    Expression of CBP IDR3 fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP IDR3 (aa 441-587) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the IDR3 region (aa 441 - 587); inse…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHR-AL-mCh-Cry2WT-NLS

    Plasmid

    #221924

    Purpose

    Expression of CBP autoregulatory loop (AL) fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP AL (aa 1529-1607) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the autoregulatory loop (AL) (aa 152…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR-IDR7-mCh-Cry2WT-NLS

    Plasmid

    #221922

    Purpose

    Expression of CBP IDR7 fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP IDR7 (aa 2109-2434) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the IDR7 region (aa 2109 - 2434); in…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR-IDR6-mCh-Cry2WT-NLS

    Plasmid

    #221921

    Purpose

    Expression of CBP IDR6 fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP IDR6 (aa 1856-2090) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the IDR6 region (aa 1856-2090); inse…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHR-IDR1-mCh-Cry2WT-NLS

    Plasmid

    #221918

    Purpose

    Expression of CBP IDR1 fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP IDR1 (aa 72-175) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the IDR1 region (aa 72 - 175); inser…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid

    #219653

    Purpose

    General mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark Venus

    Depositor

    Thomas Oertner

    Insert

    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    

    Use

    AAV

    Tags

    2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)

    Expression

    Mammalian

    Mutation

    Promoter

    nEf Addgene # 149296

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mTB3-FLAG tag

    Plasmid

    #210204

    Purpose

    Type 3 phage-display vector that contains kanamycin resistance and displays the FLAG-tag at the N-terminus of mature protein III.

    Depositor

    Brian Kay

    Article

    Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

    Insert

    FLAG-tag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mTB3-AviD tag

    Plasmid

    #210203

    Purpose

    Type 3 phage-display vector that contains kanamycin resistance and displays the AviD-tag at the N-terminus of mature protein III.

    Depositor

    Brian Kay

    Article

    Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

    Insert

    AviD-tag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge