We narrowed to 5,102 results for: codon optimized

1. pAAV uN2CpolyGly op GFP (NIID)

   Plasmid

   #224356

   Purpose

   Express GFP tagged upsteram ORF of NOTCH2NLC with an expansion (100x) of GGC repeats with codon optimization (NOT pure GGC repeats)

   Depositor

   Nicolas Charlet-Berguerand

   Article

   Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

   Insert

   uN2C with a GGC repeat expansion (100x)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Codon-optimized for expression in human, so no pu…

   Promoter

   CMV + chimeric intron (CAG)

   Available Since

   Feb. 5, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pLenti CMV Puro DEST (w118-1) SDC3

   Plasmid

   #107501

   Purpose

   Expresses SDC3, puromycin resistant

   Depositor

   Alejandro Gutierrez

   Article

   Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

   Insert

   SDC3 (codon optimized) (SDC3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Codon optimized sequence

   Promoter

   CMV

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)

   Plasmid

   #114073

   Purpose

   T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
   

   Tags

   NLS(nucleoplasmin) and 6xHis

   Expression

   Bacterial

   Mutation

   E174R, S542R, K548R and N282A

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTol2-UAS-zArchon1-KGC-EGFP-ER2

   Plasmid

   #108427

   Purpose

   Codon-optimized Archon1 fluorescent voltage reporter in zebrafish expression plasmid

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   zArchon1-KGC-EGFP-ER2
   

   Use

   Zebrafish

   Promoter

   UAS

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)

   Plasmid

   #114084

   Purpose

   CAG promoter expression plasmid for human codon optimized enAsBE1.4

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4)
   

   Tags

   2x SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R and D908A, human codon optimi…

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCJ208

   Plasmid

   #162681

   Purpose

   Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Replacement of W185-F191 with G251-T472 from MHET…

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)

   Plasmid

   #114082

   Purpose

   CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
   

   Tags

   2x SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R and D908A, human codon optimi…

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)

   Plasmid

   #114081

   Purpose

   CAG promoter expression plasmid for human codon optimized enAsBE1.1

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1)
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R and D908A, human codon optimi…

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCJ209

   Plasmid

   #162682

   Purpose

   Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Replacement of G251-T472 with W185-F191 from PETa…

   Promoter

   T7/lac

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti CMV Puro DEST (w118-1) MXRA7

    Plasmid

    #107500

    Purpose

    Expresses MXRA7, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    MXRA7 (codon optimized) (MXRA7 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Codon optimized sequence

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PET.SUMO eIF4G1 1-200

    Plasmid

    #158791

    Purpose

    Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform lacking the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1 (codon optimized)
    

    Tags

    6xHIS and SUMO

    Expression

    Bacterial

    Mutation

    amino acids 1-200

    Promoter

    T7

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTol2-UAS-zArchon2-KGC-EGFP-ER2

    Plasmid

    #108428

    Purpose

    Codon-optimized Archon2 fluorescent voltage reporter in zebrafish expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    zArchon2-KGC-EGFP-ER2
    

    Use

    Zebrafish

    Promoter

    UAS

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-28b-T7-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1931)

    Plasmid

    #114075

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
    

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    E174R, S542R, K548V and N552R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PET.SUMO eIF4G1 +MIC 1-200

    Plasmid

    #158792

    Purpose

    Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform including the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1 (with microexon) codon optimized
    

    Tags

    6xHIS and SUMO

    Expression

    Bacterial

    Mutation

    amino acids 1-200

    Promoter

    T7

    Available Since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti CMV Puro DEST (w118-1) FITM2

    Plasmid

    #107504

    Purpose

    Expresses FITM2, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    FITM2 (codon optimized)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Codon optimized sequence

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCJ200

    Plasmid

    #162673

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    S136C and a sequence Y75-F104 to introduce a 6th…

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCJ210

    Plasmid

    #162683

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    S136C, G489C, S530C, and a sequence Y75-F104 to i…

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCJ220

    Plasmid

    #162686

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224W and C529S mutations from PETase active site.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with lid deletion and C224W and C529S mutations
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    deltaG251-T472, C224W, C529S; codon optimized for…

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCJ221

    Plasmid

    #162687

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    deltaG251-T472, C224H, C529F; codon optimized for…

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET32bplus_trxAChEwt

    Plasmid

    #83916

    Purpose

    Encodes an E. coli codon optimized version of wild type human acetylcholinesterase

    Depositor

    Sarel Fleishman

    Article

    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:

    Insert

    Short form of hAChE, codon optimised for E.coli expression (ACHE Synthetic, Human)
    

    Tags

    His, S-tag, and TRX

    Expression

    Bacterial

    Mutation

    codon optimised for E.coli expression

    Promoter

    T7 promoter

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge