We narrowed to 2,414 results for: organization Addgene

1. PhyB-mCherry-MYO1

   Plasmid

   #51570

   Purpose

   Constitutively express myosin ring localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   MYO1 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. ZJOM12

   Plasmid

   #133638

   Purpose

   Integration of GFP-TLG2 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   pCUP1-GFP-Tlg2 (TLG2 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLSU4mg10

   Plasmid

   #126079

   Purpose

   Plant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cells

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Insert

   mitochondria Rieske iron sulphur protein (1-100 aa)
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Mutation

   Promoter

   'long' CaMV35S

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Rat SERCA3 (pMT2)

   Plasmid

   #75185

   Purpose

   Mammalian expression of ATP2A3

   Depositor

   Jonathan Lytton, Gary Shull

   Article

   Burk et al J Biol Chem. 1989 Nov 5;264(31):18561-8.

   Insert

   ATP2A3 (Atp2a3 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   adenovirus major late promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. ZJOM8

   Plasmid

   #133671

   Purpose

   Integration of SNF7-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on endosomes and vacuolar surface.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-SNF7-GFP (SNF7 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLaNGo11-3

   Plasmid

   #126054

   Purpose

   A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   chloroplastic FNR transit peptide
   ccdB cassette (BsaI)
   GFP11x3 (5 aa linker)
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMiNGo11-1

   Plasmid

   #126056

   Purpose

   A self-assembling split-GFP based Golden Gate vector to determine protein targeting to mitochondria in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   mitochondria Rieske iron sulphur protein (1-100 aa)
   ccdB cassette (BsaI)
   GFP11
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-GFP (F64L, S65T, V163A)-His3MX6

   Plasmid

   #53185

   Purpose

   chromosomal tagging with stable GFP

   Depositor

   John Pringle

   Article

   Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

   Insert

   HIs3MX6 (HIS3 Budding Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   GFP (F64L, S65T, V163A)

   Expression

   Mutation

   Promoter

   Available since

   May 27, 2014

   Availability

   Academic Institutions and Nonprofits only

9. JDW 1043 (pLenti6.2_CMV_V5_LEF1_P2A_H2A_mCherry)

   Plasmid

   #233538

   Purpose

   A CMV driven human LEF1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   LEF1 (LEF1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. JDW 1051 (pME_V5_GATA2_P2A_H2A_mCherry)

    Plasmid

    #232128

    Purpose

    A middle entry gateway clone containing V5 tagged human GATA2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. JDW 1031 (pME_V5_LEF1_P2A_H2A_mCherry)

    Plasmid

    #232123

    Purpose

    A middle entry gateway clone containing V5 tagged human LEF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    LEF1 (LEF1 Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. JDW 1052 (pME_V5_MAFB_P2A_H2A_mCherry)

    Plasmid

    #232129

    Purpose

    A middle entry gateway clone containing V5 tagged human MAFB and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    MAFB (MAFB Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. JDW 1060 (pME_V5_FOXF2_P2A_H2A_mCherry)

    Plasmid

    #232131

    Purpose

    A middle entry gateway clone containing V5 tagged human FOXF2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    FOXF2 (FOXF2 Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. JDW 1059 (pME_V5_GATA4_P2A_H2A_mCherry)

    Plasmid

    #232130

    Purpose

    A middle entry gateway clone containing V5 tagged human GATA4 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    GATA4 (GATA4 Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pACEBac1_Strep-Psc-BICD2(711-800)_L786A

    Plasmid

    #228838

    Purpose

    Insect cell expression of the mutant C-terminus of BICD2

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    BICD2 (Bicd2 Mouse)
    

    Use

    Tags

    Strep

    Expression

    Insect

    Mutation

    aa 711-800, with L786A

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACEBac1_Strep-Psc-BICD2(711-800)

    Plasmid

    #228833

    Purpose

    Insect cell expression of CC3 domain of BICD2 (aa 711-800)

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    BICD2 (Bicd2 Mouse)
    

    Use

    Tags

    Strep

    Expression

    Insect

    Mutation

    aa 711-800

    Promoter

    Available since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pACEBac1_Strep-Psc-BICD2(342-800)

    Plasmid

    #228832

    Purpose

    Insect cell expression of C-terminal portion of BICD2 (aa 342-800)

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    BICD2 (Bicd2 Mouse)
    

    Use

    Tags

    Strep

    Expression

    Insect

    Mutation

    aa 342-800

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pACEBac1_Strep-Psc-BICD2(711-800)_L742A

    Plasmid

    #228839

    Purpose

    Insect cell expression of the mutant C-terminus of BICD2

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    BICD2 (Bicd2 Mouse)
    

    Use

    Tags

    Strep

    Expression

    Insect

    Mutation

    aa 711-800, with L742A

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMRX-IPU-GFP-Rab5

    Plasmid

    #228139

    Purpose

    Expresses GFP-Rab5 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    Rab5 (Rab5a Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMRX-IPU-SNAP-Omp25

    Plasmid

    #228137

    Purpose

    Expresses SNAP-Omp25 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

    Insert

    Omp25 (Synj2bp Rat)
    

    Use

    Retroviral

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    transmembrane region (C-terminus)

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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