We narrowed to 2,603 results for: ubiquitin

1. pOPINK-ALG13 (OTU, aa 216-353)

   Plasmid

   #61414

   Purpose

   Expresses human ALG13 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   ALG13 (Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13) (ALG13 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-215 and aa 354-1137.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFastBac1-NEDD4L_W2-W3-W4-HECT (349)

   Plasmid

   #187721

   Purpose

   Express GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 349-975 (NEDD4L Human)
   

   Use

   Tags

   GST-HRV 3C site

   Expression

   Insect

   Mutation

   deletion of residues 1-348

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT

   Plasmid

   #187723

   Purpose

   Express His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 1-418+582-975 (NEDD4L Human)
   

   Use

   Tags

   His-TEV

   Expression

   Insect

   Mutation

   deletion of residues 419-581

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastBacHTA-NEDD4L_W1-L-W2-HECT

   Plasmid

   #187724

   Purpose

   Express His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 153-418+582-975 (NEDD4L Human)
   

   Use

   Tags

   His-TEV

   Expression

   Insect

   Mutation

   deletion of residues 1-152 and 419-581

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. His-Precission-NEDD4L (381-975)_pcDNA3.1(+)

   Plasmid

   #187725

   Purpose

   Express his-tagged NEDD4L W2-W3-W4-HECT (381) in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 381-975 (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   deletion of residues 1-380

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. His-Precission-NEDD4L (492-975)_pcDNA3.1(+)

   Plasmid

   #187726

   Purpose

   Express his-tagged NEDD4L W3-W4-HECT in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 492-975 (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   deletion of residues 1-491

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+)

   Plasmid

   #187727

   Purpose

   Express his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L with residues 226-382 removed (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   deletion of residues 226-382

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+)

   Plasmid

   #187728

   Purpose

   Express his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L with residues 418-495 removed (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   deletion of residues 418-495

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)

   Plasmid

   #187708

   Purpose

   Express his-tagged NEDD4L W2-W3-W4-HECT in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 381-975 (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-380

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a(+)-NEDD4L_W3-W4-HECT (492)

    Plasmid

    #187709

    Purpose

    Express his-tagged NEDD4L W3-W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-491

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a(+)-NEDD4L_W3-W4-HECT_C874only

    Plasmid

    #187711

    Purpose

    Express his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-975 with mutations (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-491; mutations C702A, C776…

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX6P1-NEDD4L_FL-CS

    Plasmid

    #187713

    Purpose

    Express the catalytically inactive GST-tagged NEDD4L FL in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L FL with C874 mutated to a serine (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Bacterial

    Mutation

    Cys874 mutated to a serine

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX6P1-NEDD4L_Δ1,2-linker

    Plasmid

    #187714

    Purpose

    Express GST-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L with residues 226-382 removed (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 226-382

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX6P1-NEDD4L_Δ2,3-linker

    Plasmid

    #187715

    Purpose

    Express GST-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L with residues 418-495 removed (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 418-495

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX6P1-NEDD4L_W2-W3-W4-HECT (348)

    Plasmid

    #187716

    Purpose

    Express GST-tagged NEDD4L W2-W3-W4-HECT (348) in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L with residues XX (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-347

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB-PARP1–eGFP

    Plasmid

    #211552

    Purpose

    A piggyBac vector containing CMV-PARP1-eGFP-IRES-Neo cassette.

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Piggybac

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pXPR_023d-sgCiPOLR2D

    Plasmid

    #202445

    Purpose

    Expression of a CRISPRi guide targeting POLR2D

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    POLR2D gRNA (POLR2D Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQCXIH FRB Ubiqutin-BFP

    Plasmid

    #202426

    Purpose

    TagBFP FKBP-rapamycin binding (FRB) dimerization domain fused to ubiquitin

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    FKBP-rapamycin binding (FRB) dimerization domain
    

    Use

    Lentiviral

    Tags

    Ubiquitin, TagBFP

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXPR_003-sgUBR4

    Plasmid

    #202442

    Purpose

    Expression of a guide RNA targeting UBR4

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    UBR4 gRNA (UBR4 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXPR_003-sgUBA6

    Plasmid

    #202440

    Purpose

    Expression of a guide RNA targeting UBA6

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    UBA6 gRNA (UBA6 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge