We narrowed to 2,735 results for: ubiquitin

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PHAGE-P CMVt N-HA hE6-AP I (AA 1-500)

Plasmid

#119312

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 1-500 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 1-500

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 1-376)

Plasmid

#119311

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 1-376 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 1-376

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 481-852)

Plasmid

#119316

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 481-852 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 481-852

Promoter

CMV

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

Plasmid

#85821

Purpose

iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Article

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

Zf Ubi::cMyc oDam_f_GFPoNLS

Use

Tol2 based vector

Tags

cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…

Mutation

L122A. Codon optimization compatible to work in M…

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pfast bac Rbx-HA

Plasmid

#51893

Purpose

Backbone plasmid is pfastBacHTa from invitrogen

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Rbx1 (RBX1 Human)

Tags

HA

Promoter

polyhedrin promoter

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-6P-1 p37 deltaUBX

Plasmid

#113504

Purpose

Bacterial expression of GST-tagged p37 lacking the UBX domain

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Mutation

delta 251-331 (stop codon behind AA250)

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_NLS~WT POLH

Plasmid

#246405

Purpose

Expresses WT POLH with an N-terminal NLS~FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS~FLAG

Expression

Mammalian

Mutation

The coding sequence for WT POLH has been optimise…

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_ NLS~K682A_K686A_K694_K709A (4KA) POLH

Plasmid

#246406

Purpose

Expresses K682A_K686A_K694_K709A POLH with an N-terminal NLS~FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K682A_K686A_K694_K709A. Coding sequence has …

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_ NLS~K682R_K686R_K69R_K709R (4KR) POLH

Plasmid

#246407

Purpose

Expresses K682R_K686R_K69R_K709R POLH with an N-terminal NLS~FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K682R_K686R_K69R_K709R. Coding sequence has …

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT POLH_ΔGG NEDD8

Plasmid

#221860

Purpose

Expresses FLAG-tagged WT POLH fused to ΔGG NEDD8 in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG and ΔGG NEDD8

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_K682A_K709A POLH

Plasmid

#221864

Purpose

Expresses POLH mutated at K682A and K709A with a FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K682A + K709A. Coding sequence has been opti…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_K682A_K686A_K694A_K709A POLH

Plasmid

#221865

Purpose

Expresses POLH mutated at K682A, K686A, K694A and K709A with a FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K682A, K686A, K694A + K709A. Coding sequence…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pMAPLe4 PCMTD1 PCMTD1(∆ESB-ASB9)

Plasmid

#246507

Purpose

Recombinant protein expression of PCMTD1(∆ESB-ASB9)

Depositor

Steven Clarke

Article

Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

Insert

Protein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

Deleted amino acids 266-340 in PCMTD1 and replace…

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a(+) PCMT1 H58 A214

Plasmid

#246496

Purpose

Recombinant protein expression of PCMT1 H58 A214

Depositor

Steven Clarke

Article

Pang et al bioRxiv [Preprint]. 2025 May 21:2025.05.

Insert

Protein-L-isoaspartate(D-aspartate) O-methyltransferase 1 (PCMT1 Human)

Tags

His-tag

Expression

Bacterial

Mutation

Threonine 58 mutated to Histidine and Valine 214 …

Promoter

T7

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NEDD4L_ΔC2 (157)

Plasmid

#187720

Purpose

Express GST-tagged NEDD4L ΔC2 (157) in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 157-975 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Insect

Mutation

deletion of residues 1-156

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBacHTA-NEDD4L_FL

Plasmid

#187722

Purpose

Express His-tagged NEDD4L FL in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL (NEDD4L Human)

Tags

His-TEV

Expression

Insect

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_FL

Plasmid

#187705

Purpose

Express his-tagged NEDD4L FL in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W4-HECT

Plasmid

#187710

Purpose

Express his-tagged NEDD4L W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 543-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-542

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_WW34

Plasmid

#187718

Purpose

Express GST-tagged NEDD4L WW34 tandem domains in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-581 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

only residues 492-581

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pmch-mTet1-ZF-CD pc3956

Plasmid

#201709

Purpose

Expresses of mcherry-tagged mTet1-ZF-CD in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1-ZF_CD (Tet1 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Zinc finger domain of Tet1 fused to its catalytic…

Promoter

CAG

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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