We narrowed to 4,242 results for: Pet2

1. pET28a-LSSmGFP-TEV-SAV

   Plasmid

   #191200

   Purpose

   Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP & Streptavidin fusion
   

   Use

   Tags

   6xHis and Streptavidin core domain, amino acids 1…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22b-T5-MfnG-TEV-His

   Plasmid

   #191114

   Purpose

   Expresses MfnG-TEV-His in E. coli

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   MfnG
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28.His.3C.86b.8D2Q.Tau2N4R

   Plasmid

   #226400

   Purpose

   Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 8D2Q mutant 2N4R tau for recombinant protein production in bacteria

   Depositor

   Bradley Hyman

   Article

   Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

   Insert

   Tau (MAPT Human, synthetic)
   

   Use

   Tags

   His.3C.86b

   Expression

   Bacterial

   Mutation

   8D2Q.Tau2N4R

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28.His.3C.86b.20D3Q.Tau2N4R

   Plasmid

   #226401

   Purpose

   Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 20D3Q mutant 2N4R tau for recombinant protein production in bacteria

   Depositor

   Bradley Hyman

   Article

   Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

   Insert

   Tau2N4R (MAPT Human, synthetic)
   

   Use

   Tags

   His.3C.86b

   Expression

   Bacterial

   Mutation

   20D3Q, Tau2N4R

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-PKP3(305-797)

   Plasmid

   #202599

   Purpose

   Expresses ARM domain of plakophilin3

   Depositor

   Tina Izard

   Article

   Gupta et al Int J Mol Sci. 2023 May 29;24(11):9458.

   Insert

   plakophilin3 (PKP3 Human)
   

   Use

   Tags

   His8 tag with precision protease cleavage site

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1 to 304

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)

   Plasmid

   #187708

   Purpose

   Express his-tagged NEDD4L W2-W3-W4-HECT in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 381-975 (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-380

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a(+)-NEDD4L_W3-W4-HECT (492)

   Plasmid

   #187709

   Purpose

   Express his-tagged NEDD4L W3-W4-HECT in bacterial cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 492-975 (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Bacterial

   Mutation

   deletion of residues 1-491

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-a(+)-6xHis-miRFP(-5)-HO1

   Plasmid

   #219424

   Purpose

   Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

   Depositor

   Allie Obermeyer

   Article

   Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

   Inserts

   miRFP682
   HO1
   

   Use

   Tags

   MGHHHHHGG

   Expression

   Bacterial

   Mutation

   Contains Ribosomal Binding Site for expression of…

   Promoter

   T7

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21a HsDHFR-bio-10His

   Plasmid

   #193883

   Purpose

   Expresses human dihydrofolate reductase (DHFR) with C-terminal AviTag (Bio) and His10 sequence

   Depositor

   James Inglese

   Article

   Rana et al Cell Chem Biol. 2024 Feb 15;31(2):221-23

   Insert

   human dihydrofolate reductase (DHFR) (DHFR Human)
   

   Use

   Tags

   His tag and biotinylation sequence

   Expression

   Bacterial

   Mutation

   Codon optimized

   Promoter

   T7

   Available since

   Oct. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-FKBP-IRSp53(pLxIS)

    Plasmid

    #221336

    Purpose

    Overexpression of IRSp53 pLxIS domain in bacteria

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    IRSp53 pLxIS domain (Baiap2 Mouse)
    

    Use

    Tags

    6xHis-4xFKBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-FKBP-MAVS(pLxIS)

    Plasmid

    #221335

    Purpose

    Overexpression of MAVS pLxIS domain in bacteria

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    MAVS pLxIS domain (Mavs Mouse)
    

    Use

    Tags

    6xHis-4xFKBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-FKBP-TRIF(Nterm)

    Plasmid

    #221334

    Purpose

    Overexpression of TRIF Nterm domain in bacteria

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    TRIF Nterm domain (Ticam1 Mouse)
    

    Use

    Tags

    6xHis-4xFKBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a-FKBP-GMIP(pLxIS)

    Plasmid

    #221337

    Purpose

    Overexpression of GMIP pLxIS domain in bacteria

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    GMIP pLxIS domain (Gmip Mouse)
    

    Use

    Tags

    6xHis-4xFKBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-SnoopTag-SpyTag-(AffiHER2)x3

    Plasmid

    #216281

    Purpose

    Three affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate Catcher

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    SnoopTag-SpyTag-(AffiHER2)x3
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6, SnoopTag, and SpyTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET24a-His6-CUbo-MCS

    Plasmid

    #212725

    Purpose

    Bacterial expression of a protein of interest with N-terminal His6-CUbo-fusion, contains multiple cloning site

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET24a-His7-NUbo-MCS

    Plasmid

    #212724

    Purpose

    Bacterial expression of a protein of interest with N-terminal His7-NUbo-fusion, contains multiple cloning site

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET24a-NUbo-MCS-His6

    Plasmid

    #212723

    Purpose

    Bacterial expression of a protein of interest with N-terminal NUbo- and C-terminal His6-fusions, contains multiple cloning site

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28-Flag-RBM24-95~236

    Plasmid

    #208634

    Purpose

    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236

    Depositor

    Quanfu Ma

    Insert

    human RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    only aa 95~236, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-mEGFP(A206K)-TwStrep

    Plasmid

    #208622

    Purpose

    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone

    Depositor

    Quanfu Ma

    Insert

    mEGFP(A206K)
    

    Use

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Mutation

    (compared with pEGFP-C1, protein level) A206K

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET24a-His7-NUbo-GFP

    Plasmid

    #212719

    Purpose

    Bacterial expression of His7-NUbo-GFP

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    GFP
    

    Use

    Tags

    7xHis-NUbo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge