We narrowed to 5,868 results for: nls

1. LITE2.0_pAAV_hSyn_TALE(Grm2)-GS-CIB1(NLS*,∆318-334)_WPRE_bGHpA

   Plasmid

   #47456

   Purpose

   LITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Grm2. Synapsin promoter for neuronal expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   TALE(Grm2)-GS-CIB1(NLS*,∆318-334)
   

   Use

   AAV; Tale

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. Padh1-OsTIR1(F74A)-NLS-TADH1-Sp.arg3+/pUC19*

   Plasmid

   #169353

   Purpose

   Expression of OsTIR1(F74A) in S. pombe from adh1 promoter, integrated at arg3-D4 locus

   Depositor

   Tony Carr

   Insert

   OsTIR1(F74A)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   F74A

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

   Plasmid

   #108769

   Purpose

   Expresses N-terminus to aa396 of Flp recombinase fused to ABI CID

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Insert

   Flp recombinase fragment N-terminus to aa396
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   ABI,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CAG-EGFPnls barcode-206-SV40 polyA

   Plasmid

   #190875

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFPnls-barcode206
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEM228 - [Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR]

   Plasmid

   #159895

   Purpose

   Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

   Insert

   Pmlc-1 | mNeonGreen(2x NLS) | cbr-tbb-2 UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJB003 ZF1/2x6-C 3xFLAG-NLS-VP16-FRB

   Plasmid

   #161546

   Purpose

   Inducible expression of 3xFLAG-NLS-VP16-FRB under the ZF1/2x6-C promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-VP16-FRB
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   ZF1/2x6-C

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMEH294 NLS dTomato 1x high affinity MS2 loop

   Plasmid

   #168213

   Purpose

   Mammalian expression of nuclear-localized dTomato with 1 high affinty MS2 loop

   Depositor

   Joshua Leonard

   Article

   Hung et al J Extracell Vesicles. 2016 May 13;5:3102

   Insert

   NLS dTomato
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBW2655_pCAG-PV1-NTD-ENTRY-L1-pMag-NLS-BGHpA

   Plasmid

   #108848

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to pMag/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   pMag,NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p4949 pLPCX-His-Xpress NLS hBrd4 CTD

   Plasmid

   #14459

   Depositor

   Peter Howley

   Article

   You et al Cell. 2004 Apr 30. 117(3):349-60.

   Insert

   Brd4 CTD (BRD4 Human)
   

   Use

   Retroviral

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   amino acids 1047-1362 of Brd4 (the CTD). Function…

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH

    Plasmid

    #112212

    Purpose

    Targeted DNA methylation

    Depositor

    Xingxu Huang

    Article

    Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

    Insert

    N-terminus of dCas9 and DNMT3A (DNMT3A Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    H840A,N863A

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101107

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    BDKRB2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBW2653_pCAG-PV1-NTD-ENTRY-L1-nMag-NLS-BGHpA

    Plasmid

    #108846

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to nMag/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    nMag,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSLIK mCh-V5-NES-EGFP-ERBB2 NLS zeo

    Plasmid

    #192338

    Purpose

    Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-ERBB2 NLS

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    mCh-V5-NES-EGFP-ERBB2 NLS (ERBB2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-puro-BGHpA-TRE-pEF-tagRFP-T-NLS

    Plasmid

    #163083

    Purpose

    Donor vector for integration of TagRFP-T reporter at AAVS1 safe harbor site via TALENs

    Depositor

    Lacra Bintu

    Article

    Van et al Nat Commun. 2021 Jan 22;12(1):537. doi:

    Insert

    TagRFP-T
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-c-Myc NLS-FKBP-Venus N-T2A-mRFP

    Plasmid

    #100979

    Purpose

    Encodes half of a protein delivery sensor ( NLS-FKBP-VN-T2A-RFP) for expression in mammalian cells

    Depositor

    Heinrich Leonhardt

    Article

    Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

    Insert

    c-Myc NLS-FKBP-Venus N-T2A-mRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSEM232 - [Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR]

    Plasmid

    #159898

    Purpose

    Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

    Insert

    Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBW2660_pCAG-PV1-iCre-N270-L1-FRB-NLS-BGHpA

    Plasmid

    #108737

    Purpose

    Expresses N-terminus to aa270 of Cre recombinase fused to FRB CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment N-terminus to aa270
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    FRB,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBW2549_pCAG-PV1-VCre-N269-L1-GID1-NLS-BGHpA

    Plasmid

    #108773

    Purpose

    Expresses N-terminus to aa269 of VCre recombinase fused to GID1 CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment N-terminus to aa269
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GID1,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBW2569_pCAG-PV1-GAI-L1-VCre-270C-NLS-BGHpA

    Plasmid

    #108774

    Purpose

    Expresses aa270 to C-terminus of VCre recombinase fused to GAI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    VCre recombinase fragment aa270 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    GAI and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBW1312_pCAG-PV1-NTD-ENTRY-L1-ABI-NLS-BGHpA

    Plasmid

    #108840

    Purpose

    Insert protein fragment into MluI/BspEI to fuse to ABI/NLS tags on C-terminus

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    ABI,NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge