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Plasmid#208279PurposeExpresses BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.DepositorInsertBcLOV-mCh-SOS
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB80-iLID-mCherry-RAB5
Plasmid#174624PurposeOptogenetic coupling to early endosomes (RAB5) via iLIDDepositorInsertiLID-mCherry-RAB5 (RAB5A Human, Synthetic)
UseTagsiLID-mCherryExpressionMammalianMutationmCherry: Met1Del; RAB5: Met1DelPromoterChicken beta-actinAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_EcSSPB-nano_X
Plasmid#131212PurposeExpresses EcSSPB-nano (Escherichia coli) followed by restriction sitesDepositorInsertEcSSPB-nano
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Syn:eGFP-CaM-uTEV1Δ(220-242)
Plasmid#135463PurposeNeuronal expression of uFLARE protease componentDepositorInserteGFP-CaM-uTEV1Δ
UseAAVTagseGFP, V5ExpressionMammalianMutationS219V mutation improves stability and S153N impro…PromoterSynapsinAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
IDP466
Plasmid#87008Purposegenomic Sindbis virus plasmid for expression of SYNseq componentsDepositorInsertsmyc-3xFlag-CLIP-Nrx1B-1xλN(i)
eGFP
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRMH120
Plasmid#40593PurposeExpresses Gal4BD-CRY2PHR and Gal4AD-CIB1 for light-inducible protein expressionDepositorUseTagsExpressionYeastMutationPromoterADH1 and TEFAvailable sinceOct. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pT2ADW_2paRAF
Plasmid#129654PurposeFor generating Tg mice expressing the 2P-activatable RAF system with P2A and full-length RAF1DepositorInsert2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-b2AR-hLOV1-TEVcs-FLAG-GAL4-V5
Plasmid#104842Purposeb2AR-SPARK TF componentDepositorInserthLOV1-TEVcs-FLAG-GAL4-V5
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQP-2460
Plasmid#138973PurposeLight-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1DepositorInsertNcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lyn-tdnano-HA
Plasmid#136512PurposeMammalian expression of a tandem dimer of WT SspB (nano), targeted to membrane by Lyn myristoylation sequence, with triple-HA epitope tagDepositorInsertLyn nano nano 3HA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHES609 [prNOp7-PBAC]
Plasmid#103017PurposeA S. cerevisiae plasmid. A photosensitive adenylate cyclase that produces cAMP in response to blue light. Used to activate PKA.DepositorInsertbPAC
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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IDP425
Plasmid#87022Purposegenomic Sindbis virus plasmid for use in SYNseq PLA screenDepositorInsertsSNAP-Nlg1AB-1xλN(i)
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
jk107
Plasmid#87007Purposeplasmid for cell culture expression of SYNseq componentsDepositorInsertsSNAP-Nlg1AB-1xλN(i)
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-MP-HRW-CRY2-VHH(GFP)
Plasmid#126024Purposeexpress RFP-tagged LARIAT for optogenetic clusteringDepositorInsertCIBN-MP; CRY2 PHR-VHH(GFP)
UseTagsRFPExpressionInsectMutationWTPromoterHsp70Available sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNS3
Plasmid#131770PurposePADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sitesDepositorInsertPADH1_FUSN_Fusion Red_Cry2Olig_TACT1
UseIntegration into δ-sitesTagsExpressionYeastMutationE490G to make Cry2 into Cry2OligPromoterPADH1Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNS1
Plasmid#131769PurposePADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sitesDepositorInsertFUSN_Fusion Red_Cry2WT
UseIntegration into δ-sitesTagsExpressionYeastMutationPromoterPADH1Available sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
IDP427
Plasmid#87021Purposegenomic Sindbis virus plasmid for use in SYNseq PLA screenDepositorInsertsSNAP-Nlg1AB-tdMS2(i)
mCherry
UseSynthetic Biology; Sindbis virus genomic plasmidTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMTG02-Amp-FRT
Plasmid#128271PurposePlasmid that produces the following fusion protein: TetR-LOV-RRRG(Degron)DepositorInsertTetR-LOV-RRRG
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_2pabPAC
Plasmid#158968Purpose2P-activatable bPAC for transient expressionDepositorInsert2pabPAC
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only