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pLenti-gRNA7-3

Plasmid

#248531

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA7-3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA2-2

Plasmid

#248524

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA2-2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA3-1

Plasmid

#248526

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA3-1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA5-3

Plasmid

#248528

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA5-3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA1-4

Plasmid

#248523

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA1-4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA10-3

Plasmid

#248536

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA10-3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA11-1

Plasmid

#248537

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA11-1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-gRNA11-2

Plasmid

#248538

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA11-2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-gRNA12-2

Plasmid

#248539

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA12-2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA8-2

Plasmid

#248532

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA8-2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA9-1

Plasmid

#248533

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA9-1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-gRNA9-3

Plasmid

#248534

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA9-3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA19-3

Plasmid

#248547

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA19-3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-dCas9

Plasmid

#248566

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

dCas9

Use

CRISPR

Tags

HA, 2xNLS

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CMV, TetO2

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-Pgc-1alpha-EYFP

Plasmid

#241793

Purpose

Expression vector for mouse Pgc-1alpha with EYFP

Depositor

Mary Kay Lobo

Article

Chandra et al Biol Psychiatry. 2017 Apr 1;81(7):564-57

Insert

PGC-1alpha (Ppargc1a Mouse)

Use

AAV

Tags

EYFP

Promoter

Ef1a

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pFB-EF1a-DIO-Rpl22l1-3xFLAG-2A-GFP

Plasmid

#245377

Purpose

Cre-dependent expression of FLAG-tagged Rpl22l1 and EGFP.

Depositor

D. James Surmeier

Article

Geibl et al Mol Neurodegener. 2024 Oct 8;19(1):69. d

Insert

Rpl22l1 (Rpl22l1 Mouse)

Use

AAV

Tags

3xFLAG, T2A-GFP

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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TAPBPR S104F K211L R270Q

Plasmid

#245834

Purpose

expresses TAPBPR-S104F-K211L-R270Q in S2 cells

Depositor

Nikolaos Sgourakis

Article

Sun et al Sci Adv. 2023 Feb 24;9(8):eade7151. doi:

Insert

TAPBPR (S104F K211L R270Q) (TAPBPL Human)

Tags

AviTag biotinylation tag and His6 affinity tag

Expression

Insect

Mutation

S104F K211L R270Q (HiFi mutants) & C94S (remo…

Promoter

MT_puro

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pT3TS-RfxCas13d-hei

Plasmid

#234485

Purpose

Plasmid to carry out IVT of RfxCas13d with myc tag and two oNLS (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Article

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

RfxCas13d

Use

CRISPR

Tags

myc and oNLS

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-MYC-GFP-IRES-mCherry

Plasmid

#231232

Purpose

MYC stability reporter construct (expressing c-myc 2) for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MYC (MYC Human)

Expression

Mammalian

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-MCRS1-GFP-IRES-mCherry

Plasmid

#231231

Purpose

MCRS1 stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

Depositor

Michael Rape

Article

Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.

Insert

MCRS1 (MCRS1 Human)

Expression

Mammalian

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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EIF2B3-C424G

Plasmid

#172091

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Expression

Mammalian

Mutation

c.1270T>G

Promoter

CMV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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EIF2B3-V398fs

Plasmid

#172090

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Expression

Mammalian

Mutation

c.1193-1194delTG

Promoter

CMV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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EIF2B3-S369fs

Plasmid

#172089

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Expression

Mammalian

Mutation

c.1106_1113del

Promoter

CMV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-I346T

Plasmid

#172088

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Expression

Mammalian

Mutation

c.1037T>C

Promoter

CMV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-H341Q

Plasmid

#172087

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Expression

Mammalian

Mutation

c.1023T>G

Promoter

CMV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-A322G

Plasmid

#172086

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Expression

Mammalian

Mutation

c.965C>G

Promoter

CMV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-R312Q

Plasmid

#172085

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Expression

Mammalian

Mutation

c.935G>A

Promoter

CMV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-Q236E

Plasmid

#172084

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Expression

Mammalian

Mutation

c.706C>G

Promoter

CMV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-R225Q

Plasmid

#172083

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Expression

Mammalian

Mutation

c.674G>A

Promoter

CMV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
EIF2B3-L168P

Plasmid

#172082

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Expression

Mammalian

Mutation

c.503T>C

Promoter

CMV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge