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Plasmid#138515PurposeP3fDepositorInsertT7-RNAP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTK105
Plasmid#59562PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn EYFP-PESTDepositorInsertSGP(14) 2xK-turn EYFP-PEST
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
mUAVbla:tetR 5′
Plasmid#149379PurposemUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangsDepositorInserttetA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM0553
Plasmid#128989PurposeLEU2 integration vector of pZF(3BS)-mRUBY2DepositorInsertLeu2 5' homology-pZiF promoter-mRuby2-tADH1-Con1-LEU2-Leu2 3' homology
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
CP2-TCC
Plasmid#179679PurposeComplementary plasmidDepositorInsert[sd2] T7 RNAP-TCC linker-degron
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p1.2-Neo
Plasmid#162739PurposeProtein expression in CHO cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCHO Elongation factor 1AAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC-DEST-R1R2
Plasmid#141056PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA1-MS2-PP7
Plasmid#75392PurposesgRNA1-MS2-PP7DepositorInsertsgRNA1-2XMS2-PP7
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterU6Available sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmIL-6 -231
Plasmid#61287Purposedrives luciferase via mouse IL-6 promoter, truncated (-231)DepositorInsertTruncated IL-6 promoter
UseLuciferaseTagsExpressionMammalianMutationDeletion of 1046 nucleotides from the 5' end…PromoterIL-6 promoter (truncated)Available sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 WWP1
Plasmid#67125PurposeStandard for cell-free expression benchmarking.DepositorInsertWWP1 (WWP1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWH-DI
Plasmid#47385PurposeExpresses dxs and idi under PxylA promoter (inducible by xylose) in bacteriaDepositorInsertsdxs
idi
UseTagsExpressionBacterialMutationPromoterPxylAAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB4666
Plasmid#106144PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB4848
Plasmid#106138PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB2188
Plasmid#67296PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.DepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMK376 (Hygro-P2A-mClover)
Plasmid#121187PurposeN-terminal taggingDepositorInsertHygro-P2A-mClover
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
GWF094
Plasmid#133616PurposeNS3aH1-mCherryDepositorInsertNS3aH1-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
p1.2-Hygro
Plasmid#162741PurposeProtein expression in CHO cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCHO Elongation factor 1AAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G02
Plasmid#67136PurposeGateway destination vector for cell-free expression with C-term 8xHis tagDepositorTypeEmpty backboneUseCell-free expressionTagsHisExpressionMutationPromoterAvailable sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
GWF196
Plasmid#133613PurposeNS3aH1-VPRDepositorInsertNS3aH1-VPR
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACBB-eGFP
Plasmid#32551DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionMutationPromoterConsitutive lacAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNB648
Plasmid#60140PurposeNanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter.DepositorInsertNLuc
UseTagsNuclear localization signal (NLS)ExpressionYeastMutationPromoterMET17prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-sfGFP
Plasmid#180757PurposepET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
LV anti-FITC-LaG16-R103A-T2A-BFP
Plasmid#194935PurposeLV construct encoding R103A mutant sNRR-based SynNotch receptorDepositorInsertantiFITC-LaG16-sNRR(R103A)-SynNotch-Gal4-VP64-T2A-BFP
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_hi
Plasmid#128850PurposeHigh copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTW194b63
Plasmid#136930PurposeDirected evolution of SpCas10DepositorInsertnpuC-gIII(C)
UseCRISPRTagsExpressionBacterialMutationnonePromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYL1300UaUf
Plasmid#183173PurposePlant binary vector with ccdB flanked by Ua and Uf unique nucleotide sequences.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAtU6-sgRNA
Plasmid#119775PurposeExpression of a target sgRNA in potatoDepositorInsertAtU6-SmR-sgRNA
UseTagsExpressionPlantMutationPromoterAtU6Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKSB-611U6-sgRNA3 AGAG AACA
Plasmid#173673PurposeTo clone and express a gRNA in AnophelesDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB2337
Plasmid#67555PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 POLD1
Plasmid#67128PurposeStandard for cell-free expression benchmarking.DepositorInsertPOLD1 (POLD1 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCoofy50
Plasmid#55189PurposeMammalian expression vector for parallel SLIC cloning containing N-terminal HA, 8x His and FLAG tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTags8x His, FLAG, HA, PreScission site (3C protease s…ExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_human_spike-in
Plasmid#125152PurposeEmpty vector to clone and measure the expression of promoter candidates (spike-in) by determining the abundance of transcripts originating from the candidate in human cellsDepositorTypeEmpty backboneUseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
LV TRE-GFP-TMD-DLL1
Plasmid#194936PurposeLV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.DepositorInserteGFP-(PDGFR)TMD-rDLL1 ICD (WT)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTK298
Plasmid#59567PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4DepositorInsertSGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG APX W41F dimeric (mito matrix)
Plasmid#40307DepositorInsertpea APX W41F (dimeric)
UseTagsFlagExpressionMutationW41F mutation.PromoterCAGAvailable sinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPTD-DRBD
Plasmid#35622DepositorInsertPKR DRBD-1
UseTags6xHIS, HA, and TATExpressionBacterialMutationPromoterT7Available sinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 RAD54B
Plasmid#67124PurposeStandard for cell-free expression benchmarking.DepositorInsertRAD54B (RAD54B Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TFAP2B
Plasmid#67092PurposeStandard for cell-free expression benchmarking.DepositorInsertTFAP2B (TFAP2B Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-Q
Plasmid#64980PurposeExpresses the E108Q mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E108QPromoterAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEasy Flox
Plasmid#11725DepositorInsertpEasyFlox
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only