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pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

Plasmid

#231345

Purpose

Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7/T3-BC(p1-14)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-CAG-EGFP-W3SL-BC(p1-10)

Plasmid

#231347

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3-HA_full-length

Plasmid

#222656

Purpose

Mammalian expression vector to express full-length Regnase-3 tagged with FLAG at N-term and HA at C-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-FLAG-HA-Regnase-3 WT

Plasmid

#222651

Purpose

For in vitro transcription of mouse Zc3h12c tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Expression

Mutation

Promoter

T7

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-NKp46-Fc

Plasmid

#221857

Purpose

Express human NKp46 (full-length) with C-terminal human IgG1Fc tag in mammalian cell.

Depositor

Judy Lieberman

Article

Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

Insert

NKp46 (NCR1 Human)

Use

Tags

Human IgG1Fc tag

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

Plasmid

#218916

Purpose

Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLC025 - pL0_StopCodon (CT)

Plasmid

#203905

Purpose

MoClo compatible L0 plasmid containing a stop codon. To allow for proper fusion with CDS1ns L0 plasmids, this plasmid adds an additional glycine and serine to the C-terminal end of a CDS and then adds a stop codon to terminate translation.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

Stop codon

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN381 - pTRV2-CHLH

Plasmid

#203886

Purpose

Viral silencing plasmid targeting the CHLH gene from C. roseus. For use as a positive control for VIGS experiments.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CHLH fragment

Use

RNAi

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1243 - pL0_pT3O (pro + 5U)

Plasmid

#203891

Purpose

T3O promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

T3O promoter and 5'UTR

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pUN1198 - pL0_pD4H (pro + 5U)

Plasmid

#203894

Purpose

D4H promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

D4H promoter and 5'UTR

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gαi3-91-NLuc

Plasmid

#207150

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Tom Van Loy, Dominique Schols

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAI3 (GNAI3 Human)

Use

Tags

Expression

Mammalian

Mutation

NLuc inserted at position L(91)K

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gα13-125-NLuc

Plasmid

#207155

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2

Depositor

Tom Van Loy, Dominique Schols

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNA13 (GNA13 Human)

Use

Tags

Expression

Mammalian

Mutation

NLuc inserted at position F(125)D

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-TSAA

Plasmid

#206179

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S192A and S194A

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Expression

Mutation

S192A, S194A

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-TAAS

Plasmid

#206180

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S190A and S192A

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Expression

Mutation

S190A, S192A

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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ACVR1-TASA

Plasmid

#206181

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S190A and S194A

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Expression

Mutation

S190A, S194A

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-AcGFP-CI

Plasmid

#196849

Purpose

Expression of green fluorescent reporter AcGFP driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

AcGFP

Use

Tags

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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iCAP-BI-49-KanR

Plasmid

#203534

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

AAV-BI49 Cap

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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iCAP-BI-48-KanR

Plasmid

#203533

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

AAV-BI48 Cap

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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iCAP-BI-62-KanR

Plasmid

#203535

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

AAV-BI62 Cap

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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iCAP-BI-65-KanR

Plasmid

#203536

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

AAV-BI65 Cap

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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