We narrowed to 1,878 results for: Ipp

1. pHB1

   Plasmid

   #204668

   Purpose

   pUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructs

   Depositor

   Mark Mandel

   Article

   Burgos et al mSystems. 2020 Dec 15;5(6):e00846-20. do

   Insert

   FRT-erm-FRT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAG-loxP-3xpA-H2BsfGFP-tdTomato - linker E

   Plasmid

   #125797

   Purpose

   Histone H2B-superfolder GFP and tdTomato fused by unskippable linker

   Depositor

   Mario Capecchi

   Article

   Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

   Insert

   H2BsfGFP-tdTomato
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOZ-FH-C Bach1 K141A/K142A

   Plasmid

   #73646

   Purpose

   Retroviral expression of Bach1 K141A/K142A mutant (mlh1 binding mutant)

   Depositor

   Sharon Cantor

   Insert

   Bach1 (BACH1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and His

   Expression

   Mammalian

   Mutation

   K141A/K142A ( mlh1 binding mutant)

   Promoter

   unknown

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

4. pE-FLP

   Plasmid

   #45978

   Purpose

   Expresses yeast Flp driven by the constitutive promoter pE from phage P2

   Depositor

   Keith Shearwin, Drew Endy

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Insert

   flp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   flp driven by the constitutive promoter pE from p…

   Promoter

   pE (from phage P2)

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSB-CMV-MCS-Puro cPLA2-EGFP

   Plasmid

   #162568

   Purpose

   Used as a measure of cPLA2 activation in live cells with an EGFP tag.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

   Insert

   cytosolic phospholipase a2 (pla2g4aa Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSIJ8

   Plasmid

   #68122

   Purpose

   Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

   Depositor

   Alex Nielsen

   Article

   Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

   Inserts

   flp recombinase
   rhaS
   rhaR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhamnose

   Available Since

   Dec. 8, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. MSCV-IRES-Thy1.1 DEST

   Plasmid

   #17442

   Purpose

   Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

   Depositor

   Anjana Rao

   Article

   Wu et al Cell. 2006 Jul 28. 126(2):375-87.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEB2-mKate2

   Plasmid

   #104009

   Purpose

   Plasmid encoding mKate2

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mKate2
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP578 (R32G, K42R, V45A, …

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIVT-Flp-2A-BleoR

   Plasmid

   #232752

   Purpose

   IVT template for making bicistronic modRNA encoding Flippase and Zeocin resistance gene

   Depositor

   Kate Galloway

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Inserts

   FlpO
   BleoR
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSB-CMV-MCS-Puro dr_cPLA2-mKate2

    Plasmid

    #163844

    Purpose

    Used as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

    Insert

    cytosolic phospholipase a2 (pla2g4aa Zebrafish)
    

    Use

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2

    Plasmid

    #74010

    Purpose

    Tissue-specific knock-out in zebrafish, Cas9 and Cre driven by a UAS promoter

    Depositor

    Filippo Del Bene

    Article

    Di Donato et al Genome Res. 2016 Mar 8.

    Inserts

    5xUAS
    Cas9
    Cre
    U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSEED-LoxP-sfGFP:ALFA

    Plasmid

    #219660

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method. It is equipped with LoxP sites to remove the 3xP3-dsRED.

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP:ALFA SEED Cassette (LoxP)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-VHL wt-pBabe-puro

    Plasmid

    #19234

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5 beta1

    Plasmid

    #16042

    Depositor

    Filippo Giancotti

    Article

    Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

    Insert

    beta1 (ITGB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPIPL12-FnCas12a

    Plasmid

    #199563

    Purpose

    Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia

    Depositor

    Philippe Lambin

    Article

    Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

    Insert

    Fncas12a
    

    Use

    CRISPR and Synthetic Biology; Tetracycline induci…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    COtetR-PIPL12

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2+cPLA2-mKate2

    Plasmid

    #162068

    Purpose

    Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

    Insert

    cytosolic phospholipase a2 (pla2g4aa Zebrafish)
    

    Use

    Avian, xenopus, zebrafish

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFH2.12_GagZip-MCP-P2A-eGFP

    Plasmid

    #205527

    Purpose

    Express HIV GagZip-MCP-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    GagZip-MCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Nucleocapsid replaced with leucine zipper

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H

    Plasmid

    #187186

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSDARG00000024546 (pla2g4aa Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Y90F, V91F

    Promoter

    Available Since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2+ H2B-mCherry

    Plasmid

    #99265

    Purpose

    Contains a fusion of human histone H2B to fluorescent protein mCherry in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

    Depositor

    Philipp Keller

    Article

    Wan et al Cell. 2019 Oct 3;179(2):355-372.e23. doi

    Insert

    mCherry
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. DR274-eGFP sgRNA

    Plasmid

    #61051

    Purpose

    for generation of a eGFP specific sgRNA from a T7 promoter

    Depositor

    Filippo Del Bene

    Article

    Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

    Insert

    EGFP sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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