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AAV_U6-ωRNA*_ISDra2-TnpB-mNG

Plasmid

#212968

Purpose

AAV vector for encoding an ISDra2-TnpB driven by EFs promoter

Depositor

Chunlong Xu

Article

Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

Inserts

ISDra2-TnpB-T2A-mNG,
ωRNA*

Use

AAV and CRISPR

Tags

NLS and NLS-FLAG

Expression

Mammalian

Promoter

EF1a, T7 and U6

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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ISDra2-TnpB-mCherry-U6-ωRNA*

Plasmid

#212967

Purpose

Expression vector for expression of ISDra2-TnpB driven by chicken β-actin promoter and mCherry driven by CMV promoter

Depositor

Chunlong Xu

Article

Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

Inserts

ISDra2-TnpB-T2A-mCherry
ωRNA*

Use

CRISPR

Tags

NLS and NLS-FLAG

Expression

Mammalian

Promoter

CAG, T7 and U6

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-N-HA-Gp78

Plasmid

#123591

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

autocrine motility factor receptor (Amfr Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSinREP5-iAchSnFR

Plasmid

#140643

Purpose

Acetylcholine sensor for sindbis virus

Depositor

J. Julius Zhu

Article

Borden et al bioRxiv 2020

Insert

Ach sensor V9

Expression

Mammalian

Promoter

SP6

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-hSynapsin-iAchSnFR

Plasmid

#140644

Purpose

Acetylcholine sensor for lenti virus

Depositor

J. Julius Zhu

Article

Borden et al bioRxiv 2020

Insert

Ach sensor V9

Use

Lentiviral

Promoter

hsynapsin

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-3XFLAG-Axin1

Plasmid

#123463

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

Axin 1 (Axin1 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA6-C-3XFLAG-h-Lmbr1l

Plasmid

#123462

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

Limb development membrane protein 1 like (LMBR1L Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-LB2O

Plasmid

#188036

Purpose

Fusion of human MYC Transactivation Domain (TAD) MYC Box II (MBII) with human OCT4 protein expressed in pMXs vector

Depositor

Young Tang

Article

Wang et al Stem Cell Res. 2017 Dec;25:88-97. doi: 1

Insert

MYC TAD MBII region fused with OCT4 (POU5F1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ239-RVR

Plasmid

#108863

Purpose

FnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutations

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

FnCpf1-RVR (FnCpf1 with N623R, K629R and N633R mutations)

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

N623R, K629R and N633R, Cpf1 is rice codon optimi…

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA6-C-3XFLAG-m-Lmbr1l

Plasmid

#123593

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

limb region 1 like (Lmbr1l Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-HA-m-Lmbr1l

Plasmid

#123594

Depositor

Bruce Beutler

Article

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

limb region 1 like (Lmbr1l Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX307-RAD54L2-D1-86-SFB

Plasmid

#247913

Purpose

Mammalian expression construct for C-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged RAD54L2 D1-86

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Sci Adv. 2023 Dec 8;9(49):eadi6681. doi:

Insert

RAD54L2 (RAD54L2 Human)

Use

Lentiviral

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

deletion of amino acid 1-86

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX307-RAD54L2-FL-SFB

Plasmid

#247912

Purpose

Mammalian expression construct for C-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged RAD54L2

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Sci Adv. 2023 Dec 8;9(49):eadi6681. doi:

Insert

RAD54L2 (RAD54L2 Human)

Use

Lentiviral

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX307-SFB-ZNF451

Plasmid

#247909

Purpose

Mammalian expression construct for N-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged ZNF451

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Sci Adv. 2023 Dec 8;9(49):eadi6681. doi:

Insert

ZNF451 (ZNF451 Human)

Use

Lentiviral

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-Zfr-P2A-HA-luciferase

Plasmid

#223188

Purpose

Plasmid containing the Zfr/Firefly luciferase cassette. Translation of luciferase is controlled by splicing modulation of the Zfr by risdiplam.

Depositor

Yuxuan Guo

Article

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Insert

luciferase (LOC116160065 firefly)

Use

AAV

Tags

HA and engineered P2A

Promoter

TNNT2

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-COX2-Q6-R18-G1333C-UGI-Puro

Plasmid

#198845

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX2-Q6-R18 TALEN

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ATP8-W9-R15-G1333N-UGI-Puro

Plasmid

#198841

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ATP8-W9-R15 TALEN

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ATP8-W9-L17-G1333C-UGI-Puro

Plasmid

#198840

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ATP8-W9-L17 TALEN

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-COX2-Q6-L18-G1333N-UGI-Puro

Plasmid

#198844

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX2-Q6-L18 TALEN

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKAcat(G1A)-mCherry

Plasmid

#181852

Purpose

mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Tags

mCherry

Expression

Mammalian

Mutation

Glycine residue immediately after start site in P…

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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