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We narrowed to 2,317 results for: crispr system

Showing: 1641 - 1660 of 2317 results

  1. pSL1142 (pSPIN, pBBR1 backbone)

    Plasmid
    #160730
    Purpose
    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    160730-plasmid-map-sequence-id-357931

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  2. pSL1425 (pSPIN, pCDF backbone)

    Plasmid
    #160735
    Purpose
    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    160735-plasmid-map-sequence-id-317231

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  4. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid
    #162277
    Purpose
    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.
    Depositor
    Kazuhisa Nakayama
    Article
    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi
    Insert
    HiFi Cas9
    Use
    CRISPR
    Tags
    BPNLS and FLAG tag
    Expression
    Mammalian
    Mutation
    SpCas9 (R691A)
    Promoter
    Available since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    162277-plasmid-map-sequence-id-319794

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  5. SunTagCACTA1g2-22aa-TET1cd

    Plasmid
    #106437
    Purpose
    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the CACTA1 promoter
    Depositor
    Steven Jacobsen
    Article
    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Insert
    CACTA1gRNA2_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    106437-plasmid-map-sequence-id-205837

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  6. pDrop

    Plasmid
    #184872
    Purpose
    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.
    Depositor
    Patrick Sobetzko
    Article
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    lacZalpha,ccdB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 3, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    184872-plasmid-map-sequence-id-364202

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  19. pCMV-dCas13-NES-eNAT10

    Plasmid
    #238299
    Purpose
    Expresses cytoplasmic version of the programmable RNA acetylation system.
    Depositor
    Won Do Heo
    Article
    Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/
    Insert
    dPspCas13b fused to eNAT10 (NAT10 Prevotella sp. P5-125, Human)
    Use
    CRISPR
    Tags
    HIV NES (C terminal of dPspCas13b)
    Expression
    Mammalian
    Mutation
    H133A for catalytically inactive mutant, deletion…
    Promoter
    Available since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    238299-plasmid-map-sequence-id-472414

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  20. pCMV-NLS-dCas13-NLS-eNAT10

    Plasmid
    #238300
    Purpose
    Expresses nuclear version of the programmable RNA acetylation system.
    Depositor
    Won Do Heo
    Article
    Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/
    Insert
    dPspCas13b fused to eNAT10 (NAT10 Prevotella sp. P5-125, Human)
    Use
    CRISPR
    Tags
    SV40 BPNLS and SV40 BPNLS (C terminal of dPspCas1…
    Expression
    Mammalian
    Mutation
    H133A for catalytically inactive mutant, deletion…
    Promoter
    Available since
    June 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    238300-plasmid-map-sequence-id-474324

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Showing: 1641 - 1660 of 2317 results