166,289 results

1. pCR8-PspCas13b_gRNA[ccdbCam]_1xMS2b

   Plasmid

   #196847

   Purpose

   backbone for gRNA cloning of dpspCas13b tagged by 1xMS2

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pzk408-pmax-3xflag-dpspCas13b(AAAA)-NES

   Plasmid

   #196828

   Purpose

   Express dpdpCas13b with AAAA mutation

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Insert

   dpspCas13b(AAAA)
   

   Tags

   3XFLAG and NES

   Expression

   Mammalian

   Mutation

   K367A, D369A and K370A

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKB238

   Plasmid

   #170033

   Purpose

   Encodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporter

   Depositor

   Jeffrey Tabor

   Article

   Brink et al bioRxiv

   Inserts

   mNeonGreen
   Neuropeptide Y (NPY Human, Synthetic, Escherichia coli)
   

   Use

   Synthetic Biology

   Tags

   Lpp, OmpA, and Tether

   Expression

   Bacterial

   Promoter

   Ptac and PvirK

   Available Since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHH0103_RAPGEF4_DEP

   Plasmid

   #109895

   Purpose

   Protein expression and purification of RAPGEF4_DEP

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   RAPGEF4_DEP (RAPGEF4 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6379 H10-K218T

   Plasmid

   #1250

   Depositor

   Susan Lindquist

   Article

   Glover et al Cell 1998 Jul 10;94(1):73-82.

   Insert

   Hsp104 (K218T) (HSP104 Budding Yeast)
   

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   K218T

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

6. 6380 H10-K620T

   Plasmid

   #1251

   Depositor

   Susan Lindquist

   Article

   Glover et al Cell 1998 Jul 10;94(1):73-82.

   Insert

   Hsp104 (K620T) (HSP104 Budding Yeast)
   

   Tags

   his tag

   Expression

   Bacterial

   Mutation

   K620T

   Available Since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pHAGE mNeonGreen-Core (HBc from HBV) IRES puro

   Plasmid

   #122202

   Purpose

   Plasmid coding for mNeonGreen-Core protein (from HBV AYW) and a puromycin resistance in a lentiviral backbone.

   Depositor

   Raphael Gaudin

   Article

   Hallez et al Nat Microbiol. 2019 Apr 1. pii: 10.1038/

   Inserts

   HBc from HBV AYW
   mNeonGreen
   

   Tags

   GGSGGSGGS linker

   Expression

   Mammalian

   Available Since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.CAG.GCaMP6f.WPRE.SV40

   Plasmid

   #100836

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from CAG promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV2, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

   Promoter

   CAG

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAT2

   Plasmid

   #112583

   Purpose

   Used for expression of protein in E. coli as non-cleavable minimal N-terminal 6His-tag fusions

   Depositor

   Marko Hyvönen, Johan Peränen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Tags

   6His

   Expression

   Bacterial

   Available Since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-SFFV-MCS-WPRE-UbC-MCS

    Plasmid

    #211798

    Purpose

    Lentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and another multiple cloning site (MCS) under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    multiple cloning site
    multiple cloning site
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV and UbC

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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