We narrowed to 2,359 results for: Efs

1. lentiCRISPR v2-Blast

   Plasmid

   #83480

   Purpose

   Mammalian expression of Cas9 and sgRNA scaffold

   Depositor

   Mohan Babu

   Insert

   Blasticidin S deaminase
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   EF-1α

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVP299

   Plasmid

   #222068

   Purpose

   GreenGate EF base entry vector with an Esp3I domesticated backbone.

   Depositor

   Wayne Parrott

   Article

   Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Greengate compatible cloning v…

   Tags

   Expression

   Mutation

   Domesticated of Esp3I sites

   Promoter

   Available since

   Dec. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pVP184

   Plasmid

   #222051

   Purpose

   GreenGate EF bridge module containing filler sequence.

   Depositor

   Wayne Parrott

   Article

   Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

   Insert

   Filler
   

   Use

   Synthetic Biology; Greengate compatible cloning v…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. EF1a-mEmerald-CAPRIN1

   Plasmid

   #164218

   Purpose

   Expresses mEmerald tagged CAPRIN1 under EF-1a promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   CAPRIN1 (CAPRIN1 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Expression

   Mutation

   Promoter

   EF-1a

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pInducer-myc-ERK1-R84S/S170D

   Plasmid

   #213593

   Purpose

   Doxycycline inducible expression of myc-tagged ERK1-R84S/S170D cDNA in mammilian cells

   Depositor

   Channing Der

   Article

   Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

   Insert

   MAPK3 (MAPK3 Human)
   

   Use

   Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   R84S, S170D

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p300 core-dCas9-CBP core

   Plasmid

   #179559

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) & c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, EP300 Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pInducer-myc-ERK2-R67S/S153D

   Plasmid

   #213597

   Purpose

   Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D cDNA in mammilian cells

   Depositor

   Channing Der

   Article

   Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   Lentiviral

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   R67S, S153D

   Promoter

   Available since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. GCN5 FL-dCas9-CBP core

   Plasmid

   #179556

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. GCN5 core-dCas9-CBP core

   Plasmid

   #179555

   Purpose

   encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. BAP1-C91A

    Plasmid

    #108440

    Purpose

    Stable expression of BAP1-C91A in mammalian cells.

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C91A: c.271T>G, c.272G>C

    Promoter

    CMV

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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