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pDIV272

Plasmid

#226183

Purpose

Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

tRNA-sgRNA-tRNA

Use

CRISPR

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

TEF1p (Komagataella phaffii)

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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TR2

Plasmid

#218403

Purpose

Expresses TR2 peptide in AAV9 capsid

Depositor

Casey Maguire

Article

Hanlon et al Molecular Therapy. 2024 Jun 5. doi.org/1

Insert

TR2 7mer (RTTASLM)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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NL1

Plasmid

#218404

Purpose

NL1 insert in AAV9 capsid

Depositor

Casey Maguire

Article

Hanlon et al Molecular Therapy. 2024 Jun 5. doi.org/1

Insert

NL1 7mer (LTTEGRR)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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TH1

Plasmid

#218405

Purpose

TH1 insert in AAV9 capsid

Depositor

Casey Maguire

Article

Hanlon et al Molecular Therapy. 2024 Jun 5. doi.org/1

Insert

TH1 7mer (HPARALP)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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TP1

Plasmid

#218406

Purpose

TP1 insert in AAV9 capsid

Depositor

Casey Maguire

Article

Hanlon et al Molecular Therapy. 2024 Jun 5. doi.org/1

Insert

TP1 7mer peptide (PKYPLLG)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSEVA424::tsi5

Plasmid

#192960

Purpose

Expresses the protein Tsi51-76 for biological function studies in a broad-spectrum of organisms

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tsi5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET29a(+)::D1141A

Plasmid

#192963

Purpose

Test the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGL

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

Tse5_D1141A

Use

Tags

9xhis + TEV tag

Expression

Bacterial

Mutation

D1141A

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET29a(+)::9xhis-Tse5

Plasmid

#192962

Purpose

Heterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CT

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

Tse5

Use

Tags

9xhis + TEV tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA251::PpsbA2*::B0032::CYP110D1 N-terminal His-Tag

Plasmid

#186708

Purpose

N-terminal His-tagged CYP110D1 coding sequence under the control of PpsbA2* promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

Depositor

Paula Tamagnini

Article

Mascia et al Green Chemistry, 24 (2022):6156

Insert

alr4766

Use

Synthetic Biology

Tags

His-Tag (6His)

Expression

Mutation

Promoter

PpsbA2*

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1 N-terminal His-Tag

Plasmid

#186710

Purpose

N-terminal His-tagged CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

Depositor

Paula Tamagnini

Article

Mascia et al Green Chemistry, 24 (2022):6156

Insert

alr4766

Use

Synthetic Biology

Tags

His-Tag (6His)

Expression

Mutation

Promoter

Ptrc.x.tetO2

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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