We narrowed to 6,904 results for: crispr cas9 plasmids

1. Nme2Cas9_pMCSG7

   Plasmid

   #119925

   Purpose

   Nme2Cas9 bacterial expression plasmid

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   Tags

   NLS, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nme2Cas9_pCDest

   Plasmid

   #119923

   Purpose

   Nme2Cas9 CMV-driven mammalian expression plasmid

   Depositor

   Erik Sontheimer

   Article

   Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

   Insert

   Nme2Cas9
   

   Use

   Tags

   NLS-3xHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD2-dcas9-J23101-GFP

   Plasmid

   #122260

   Purpose

   Constitutive dCas9 and GFP expression plasmid

   Depositor

   Chueh Loo Poh

   Article

   Zhang et al Sci Rep. 2018 Sep 3;8(1):13127. doi: 10.

   Insert

   dCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. cTRE-LSL-GFP-IRES-Cas9-sgCR8

   Plasmid

   #135668

   Purpose

   Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

   Depositor

   Florian Karreth

   Insert

   sgCR8
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

   Plasmid

   #135669

   Purpose

   Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

   Depositor

   Florian Karreth

   Insert

   sgPten
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX458-3xHA-SpCas9-HF1

   Plasmid

   #201952

   Purpose

   Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   Mammalian expression plasmid of high-fidelity SpCas9 variant SpCas9-HF1 with T2A-EGFP and cloning backbone for sgRNA
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA

   Plasmid

   #61591

   Purpose

   A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin

   Plasmid

   #187953

   Purpose

   FKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   FKBP12_F36V-SpdCas9-KRAB-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin

   Plasmid

   #187957

   Purpose

   FKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   FKBP12_F36V-KRAB-SpdCas9-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti SYN-SVI-DIO-dCas9-KRAB-MeCP2

    Plasmid

    #170378

    Purpose

    Expresses a SYN-driven, intron-containing dCas9-KRAB-MeCP2 fusion in which the part of the dCas9 cassette is double floxed and in inverted orientation (DIO). Requires Cre-dependent recombination.

    Depositor

    Jeremy Day

    Article

    Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

    Insert

    A DIO FLAG-dCas9-KRAB-MeCP2 cassette containing an SV40 intron
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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