We narrowed to 4,833 results for: U6...

1. pU6-RanCas13b-non-targeting (pJK6)

   Plasmid

   #229056

   Purpose

   Cloning backbone for RanCas13b crRNA (U6 promoter)

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. eSpCas9(1.1)_NoFlag_ATP1A1_G3_SLBP_G1_Dual_sgRNA

   Plasmid

   #191528

   Purpose

   Vector for tandem expression of SLBP 3'UTR G1 sgRNA in combination with ATP1A1 G3 sgRNA from two independent U6 promoters to facilitate SLBP endogenous tagging by marker free coselection using ouabain

   Depositor

   Yannick Doyon

   Insert

   SLBP 3'UTR G1 sgRNA + ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDest_U6_A2_CG2_erbb2_gRNA171

   Plasmid

   #199385

   Purpose

   pDEL133; Gateway Destination vector for cell-type specific CRISPR encoding erbb2 sgRNA

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Insert

   erbb2 sgRNA 171
   

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO.1 LARP7-FLAG m6A mut 7SK

   Plasmid

   #200734

   Purpose

   Lentiviral expression of Flag-Tagged human LARP7 and the snRNA 7SK

   Depositor

   Claudio Alarcón

   Insert

   LARP7 and 7SK (LARP7 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   7SK m6A mutations: A172G, A220T, A228G, A281G, an…

   Promoter

   U6

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. GGDestX2-Amp

   Plasmid

   #157654

   Purpose

   Golden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNA

   Depositor

   Karmella Haynes

   Article

   Haynes et al Methods Mol Biol. 2023;2599:191-214. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2

   Plasmid

   #118159

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

   Plasmid

   #118160

   Purpose

   CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS

   Depositor

   Jorge Ferrer

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p7SKU6SBpur

   Plasmid

   #160329

   Purpose

   dual sh RNA vector expressing cfSBFP2 and a puromycin selection marker

   Depositor

   Ikuo Wada

   Article

   Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

   Type

   Empty backbone

   Use

   Tags

   cfSBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiCRISPRv2R

   Plasmid

   #167932

   Purpose

   lentiCRISPRv2 variant with mScarlet-I fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p246 eSpCas9_2gRNAs_mH11

    Plasmid

    #164854

    Purpose

    gRNA vector for targeting mouse H11 locus

    Depositor

    Philip Jordan

    Article

    Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

    Insert

    gRNAs for targeting mouse H11 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti SpBsmBI sgRNA Hygro

    Plasmid

    #62205

    Purpose

    An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

    Depositor

    Rene Maehr

    Article

    Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJAT32

    Plasmid

    #204297

    Purpose

    pJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-As-crRNA

    Plasmid

    #78956

    Purpose

    Cloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. vHB16

    Plasmid

    #193455

    Purpose

    Expression of GFP-Cas9-NLS and gRNA in A. castellanii

    Depositor

    Chantal Abergel

    Article

    Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

    Insert

    tRNA-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-CHMP4B gRNA2

    Plasmid

    #211629

    Purpose

    sgRNA-2 against CHMP4B

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    sgRNA CHMP4B
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti-CHMP4B gRNA1

    Plasmid

    #211628

    Purpose

    sgRNA-1 against CHMP4B

    Depositor

    Agnel Sfeir

    Article

    Brambati et al Science. 2023 Aug 11;381(6658):653-660.

    Insert

    sgRNA CHMP4B
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSFDuet1-sgRNA(siteA)

    Plasmid

    #154831

    Purpose

    sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site A in CHO cells

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Insert

    sgRNA(siteA)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRB1017

    Plasmid

    #59936

    Purpose

    empty vector for gRNA cloning

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. zCREST3:redCas9; control_gRNA

    Plasmid

    #199334

    Purpose

    pDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    zCREST3
    Cas9
    control sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti SpBsmBI sgRNA Puro

    Plasmid

    #62207

    Purpose

    An empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)

    Depositor

    Rene Maehr

    Article

    Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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