We narrowed to 20,958 results for: ACE;

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pZA2[PTAN:GFP]

Plasmid

#60779

Purpose

Contains PTAN driving expression of GFP

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Insert

yemGFP

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pSecEffector

Plasmid

#172891

Purpose

Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Inserts

Bxb1
TP901
TEV Display cassette
ProD promoter between anti aligned Bxb1 sites

Expression

Bacterial

Promoter

ProD, nTETO, and pBAD /AraC

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET21-tVYN-Nt∆

Plasmid

#154431

Purpose

Plasmid used to express the tVYN-Nt∆ luminescent biosensor for use in live cell cyclic di-GMP detection

Depositor

Ming Hammond

Article

Dippel et al ACS Chem Biol. 2020 Mar 25. doi: 10.1021

Insert

VenusΔC12-NtYcgRΔ-NLucΔ4

Expression

Bacterial

Promoter

T7

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFTK056

Plasmid

#171328

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

SpCas9-NLS

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBbB13k-GFP

Plasmid

#126122

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

GFPuv

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23102

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pFTK088

Plasmid

#171360

Purpose

AMA1 sequence as Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

AMA1 sequence (short), entry vector providing fungal replication

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK019

Plasmid

#171291

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PTET-10x-repeat promoter+CPgpdA (fused)

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK017

Plasmid

#171289

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PTET-5x-repeat promoter+CPgpdA (fused)

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK038

Plasmid

#171310

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

hph, hygR, hygromycin selection marker

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK037

Plasmid

#171309

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

bleoR phleomycin, selection marker

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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NAT-TET-OFF -LEU- 4xFLAG

Plasmid

#182527

Purpose

Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

7 Copies of TetO Box

Tags

4XFLAG

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pFTK084

Plasmid

#171356

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

TtRNA[Met] A.niger Pol-III terminator

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK039

Plasmid

#171311

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

pyrG AO090011000868 Orotidine 5'-phosphate decarboxylase, selection marker

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK080

Plasmid

#171352

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Tcyc1 YJR048W terminator

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK079

Plasmid

#171351

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Toat1 Pc18g03600 terminator

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK052

Plasmid

#171324

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

Firefly luciferase reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBLlSh2

Plasmid

#170981

Purpose

Expression of ShCAST under control of lacI Plac promoter/repressor pair.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Inserts

lacI
ShTnsB, ShTnsC, ShTniQ, ShCas12k

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pLlac01

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pFTK049

Plasmid

#171321

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

mCherry fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pHelper_O

Plasmid

#173783

Purpose

The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

186 Integrase

Use

Synthetic Biology

Expression

Bacterial

Mutation

E59G - please see depositor comments

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHelper_W

Plasmid

#173784

Purpose

The Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

Wphi Integrase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHelper_D

Plasmid

#173781

Purpose

The D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

D145 Integrase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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msEGFP-PACR_pRSETB

Plasmid

#55775

Purpose

Genetically encoded caged Ca2+

Depositor

Takeharu Nagai

Article

Fukuda et al ACS Chem Biol. 2014 May 16;9(5):1197-203

Insert

PACR

Tags

6xHis and msEGFP

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 3, 2014

Availability

Academic Institutions and Nonprofits only
pRSET B-BKAR

Plasmid

#14877

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2005 Feb 18. 280(7):5581-7.

Insert

B kinase activity reporter

Tags

CFP and YFP

Expression

Bacterial

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pICSL30004

Plasmid

#50302

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS no stop codon, YFP variant of GFP (Aequorea victoria)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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TDGS50

Plasmid

#183189

Purpose

TEV protease expressed under J23106 promoter; DivIVAsfGFP-TEV protease recognition site-M0050 expressed under J23116 promoter

Depositor

Hsiao-Chun Huang

Article

Hong et al ACS Synth Biol. 2021 Aug 20;10(8):1830-1

Inserts

DivIVA-GFP(tevR M0050)
TEV-S219V

Expression

Bacterial

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK051

Plasmid

#171323

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

eBFP fluorescent reporter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR APLP2 shRNA 3

Plasmid

#30161

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP2 shRNA 3

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pICSL50011

Plasmid

#50317

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, EOS photoactivatable fluorescent protein (L. hemprichii)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pMulti-sgRNA-LacZ

Plasmid

#99913

Purpose

Receptor plasmid for the assembly of multiple sgRNAs

Depositor

Yujie Sun

Article

Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pUCSh3-mRFP

Plasmid

#170983

Purpose

Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

mRFP

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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