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pAV-U6+27Linear-2, PolyU(0)

Plasmid

#166994

Purpose

Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(0)

Expression

Mammalian

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-Dnase1l1l-mRFP

Plasmid

#169002

Purpose

Express zebrafish Dnase1l1l-mRFP in zebrafish

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

Dnase1l1l-mRFP (dnase1l3l Zebrafish)

Use

Unspecified

Tags

mRFP

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1, PolyU(0)

Plasmid

#166992

Purpose

Tests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(0)

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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GLY, Linear-3, PolyU(1)

Plasmid

#166988

Purpose

Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLNYtRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(1)

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pJJF144_SECGFP_sg2

Plasmid

#163865

Purpose

sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

Depositor

Nikolaus Rajewsky

Article

Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

Insert

sgRNA targeting GFP

Use

CRISPR

Expression

Worm

Promoter

R07E5.16 (U6)

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRb_candidate8

Plasmid

#165006

Purpose

Expression of candidate TCR #8 TCRbeta cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRB (TRB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRa_candidate8

Plasmid

#165005

Purpose

Expression of candidate TCR #8 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc318

Plasmid

#161941

Purpose

Carries Golden Gate substitution insert for ER-LBD domain-insertion mapping

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1A3-BbsI-G-ER-LBD-S-SapI

Expression

Bacterial

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(1)

Plasmid

#159479

Purpose

Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(1)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(2)

Plasmid

#159480

Purpose

Tests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(2)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(3)

Plasmid

#159481

Purpose

Tests for the impact of 3 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(3)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pBz118_His_CsoS2

Plasmid

#140841

Purpose

Halothiobacillus neapolitanus CsoS2 with N-terminal His-tag, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLz54_polyPro_polar

Plasmid

#140847

Purpose

Same as pLz47 but with 20 polar amino acids instead of N*- peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with polar residues

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLz58_polyPro_CsoS2_CTP

Plasmid

#140850

Purpose

Same as pLz47 but with C-terminal peptide (CTP) instead of N*- peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 C-terminal peptide

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLz55_polyPro_random_Npeptide

Plasmid

#140846

Purpose

Same as pLz47 but with randomized N*-peptide sequence

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with randomized CsoS2 N-peptide

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLz56_polyPro_CsoS2_MR_r1

Plasmid

#140848

Purpose

Same as pLz47 but with MR repeat 1 instead of N*-peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 MR repeat 1

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLz57_polyPro_CsoS2_CTD_r8

Plasmid

#140849

Purpose

Same as pLz47 but with CTD repeat 8 instead of N*-peptide

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

polyproline helix with CsoS2 CTD repeat 8

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJ211_WT_med

Plasmid

#128849

Purpose

Medium copy number plasmid for expression of E. coli flagellin from its natural promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pJ211_rha_WT_hi

Plasmid

#128853

Purpose

High copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+ AMIC

Plasmid

#118420

Purpose

For mammalian expression of Amoeba myosin 1C

Depositor

Michael Tsang

Article

Saydmohammed et al Nat Commun. 2018 Aug 23;9(1):3381. doi:

Insert

Amoeba myosin 1C

Expression

Mammalian

Mutation

See depositor comments below

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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