We narrowed to 4,221 results for: 424

1. pRS416 CUP1pro-Htt103QdeltaP125-160-YFP

   Plasmid

   #224883

   Purpose

   Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the first 36 amino acids of the proline-rich region (125-160) tagged with YFP using copper.

   Depositor

   Paul Herman

   Insert

   Htt103QdeltaP125-160 (HTT Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   deltaP125-160

   Promoter

   CUP1

   Available since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS416 CUP1pro-Htt103QdeltaP141-170-YFP

   Plasmid

   #224880

   Purpose

   Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 30 amino acids of the proline-rich region (141-170) tagged with YFP using copper.

   Depositor

   Paul Herman

   Insert

   Htt103QdeltaP141-170 (HTT Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   deltaP141-170

   Promoter

   CUP1

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS416 CUP1pro-Htt103QdeltaP161-170-YFP

   Plasmid

   #224876

   Purpose

   Low copy plasmid for inducible yeast expression of the Htt exon 1 construct without the last 10 amino acids of the proline-rich region (161-170) tagged with YFP using copper.

   Depositor

   Paul Herman

   Insert

   HTT103QdeltaP161-170 (HTT Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   delta161-170

   Promoter

   CUP1

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-IDR4-mCh-Cry2WT-NLS

   Plasmid

   #221920

   Purpose

   Expression of CBP IDR4 fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP IDR4 (aa 662-787) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the IDR4 region (aa 662-787); insert…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-CFID-mCh-Cry2WT-NLS

   Plasmid

   #221923

   Purpose

   Expression of CBP CFID fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP CFID (aa 1892-2441) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the CBP-FUS interaction domain (CFID…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-IDR3-mCh-Cry2WT-NLS

   Plasmid

   #221919

   Purpose

   Expression of CBP IDR3 fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP IDR3 (aa 441-587) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the IDR3 region (aa 441 - 587); inse…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-AL-mCh-Cry2WT-NLS

   Plasmid

   #221924

   Purpose

   Expression of CBP autoregulatory loop (AL) fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP AL (aa 1529-1607) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the autoregulatory loop (AL) (aa 152…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-IDR7-mCh-Cry2WT-NLS

   Plasmid

   #221922

   Purpose

   Expression of CBP IDR7 fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP IDR7 (aa 2109-2434) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the IDR7 region (aa 2109 - 2434); in…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR-IDR6-mCh-Cry2WT-NLS

   Plasmid

   #221921

   Purpose

   Expression of CBP IDR6 fragment fused to optogenetic protein mCh-Cry2WT

   Depositor

   Daniel Bose

   Insert

   CBP IDR6 (aa 1856-2090) (CREBBP Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   insertion of the IDR6 region (aa 1856-2090); inse…

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR-IDR1-mCh-Cry2WT-NLS

    Plasmid

    #221918

    Purpose

    Expression of CBP IDR1 fragment fused to optogenetic protein mCh-Cry2WT

    Depositor

    Daniel Bose

    Insert

    CBP IDR1 (aa 72-175) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    insertion of the IDR1 region (aa 72 - 175); inser…

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcGFP-N1_CBPdCFIDKTG

    Plasmid

    #221917

    Purpose

    Transient expression of GFP-tagged CBPdeltaCFIDKTGL in mammalian cells

    Depositor

    Daniel Bose

    Insert

    CBP (CREBBP Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deletion of the CBP-FUS interaction domain (CFID)…

    Promoter

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. RT-5

    Plasmid

    #220442

    Purpose

    Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)

    Depositor

    Marvin Wickens

    Article

    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

    Insert

    PUF3 (puf-3 Nematode)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. RT-4

    Plasmid

    #220443

    Purpose

    Construct used to generate the PUF3/PUP2/3HA tagging strains. Surrounding the PUP2/3HA/URA3 are large regions of homology to integrate at the 3’ end of the PUF3 gene.

    Depositor

    Marvin Wickens

    Article

    Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

    Insert

    PUF3 (PUF3 Budding Yeast)
    

    Use

    Tags

    3xHA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cav-B

    Plasmid

    #209879

    Purpose

    Dimerization dependent fluorescent protein B anchored to cytosolic face of the caveolae with mouse CAVIN1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Use

    Tags

    CAVIN1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-C1-hEFA6C

    Plasmid

    #206414

    Purpose

    Mammalian expression of hEFA6C

    Depositor

    Hye-Won Shin, Kazuhisa Nakayama

    Article

    Ueda et al FEBS Lett. 2013 Jun 5;587(11):1617-23. d

    Insert

    EFA6C (PSD2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKG-GW2-MaUPPS-B

    Plasmid

    #203175

    Purpose

    Yeast expression vector for accessory subunit of Methanosarcina acetivorans cis-prenyltransferase

    Depositor

    William Sessa

    Article

    Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

    Insert

    MA_4402
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKG-GW1-MaUPPS-A

    Plasmid

    #203174

    Purpose

    Yeast expression vector for catalytic subunit of Methanosarcina acetivorans cis-prenyltransferase

    Depositor

    William Sessa

    Article

    Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

    Insert

    uppS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. mouse KANK4-Y801H Ankyrin Repeats pET151

    Plasmid

    #164058

    Purpose

    Expresses mouse KANK4 Ank domain -Y801H mutant in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 4 Y801H mutant (Kank4 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3E-rab25b

    Plasmid

    #109683

    Purpose

    Multisite gateway vector for 3' tagging with rab25b

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab25b (rab25b Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. actc1b-mKate2-rab25b

    Plasmid

    #109615

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab25b

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab25b (rab25b Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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