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NW11

Plasmid

#133443

Purpose

Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW11

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-IRAlus-Nicn1

Plasmid

#92345

Purpose

Plasmid for expression of GFP gene with a Nicn1 gene derived IR Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Nicn1 gene derived IR Alu element containing 3'UTR (NICN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGG>nls-hCas9-nls

Plasmid

#99138

Purpose

Expresses Cas9 via the chicken beta actin promoter for electroporation in chicken embryos

Depositor

Marianne Bronner

Article

Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

Insert

humanized Cas9

Use

CRISPR

Promoter

Chicken beta actin (CAG)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-Alu-Nicn1

Plasmid

#92344

Purpose

Plasmid for expression of GFP gene with a Nicn1 gene derived Alu element containing 3'UTR.

Depositor

Gordon Carmichael, Ling-Ling Chen

Article

Chen et al EMBO J. 2008 Jun 18;27(12):1694-705. doi

Insert

Nicn1 gene derived Alu element containing 3'UTR (NICN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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myc-Pex19

Plasmid

#127625

Purpose

N-terminal myc-tagged mouse Pex19

Depositor

Josef Kittler

Article

Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

Insert

Pex19 (Pex19 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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NW9

Plasmid

#133442

Purpose

Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW9

Expression

Bacterial

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pMRBAD-link-CGFP

Plasmid

#52733

Purpose

L-(+)-arabinose-inducible expression of link-CGFP negative control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

link-CGFP

Expression

Bacterial

Promoter

PBAD

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET11a-link-NGFP

Plasmid

#52732

Purpose

IPTG-inducible expression of link-NGFP negative control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

link-NGFP

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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SH4-FRB-EBFP2

Plasmid

#222418

Purpose

To express a plasma membrane "anchor" for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

SH4

Tags

FRB-HA-EBFP2

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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HRE-dUnOHR

Plasmid

#201714

Purpose

Hypoxia responsive promoter driving UnaG and mOrange

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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LCAPCW

Plasmid

#205646

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

hCMV

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Lyn-tailed mCherry-SEpHluorin

Plasmid

#32002

Depositor

Sergio Grinstein

Article

Koivusalo et al J Cell Biol. 2010 Feb 22;188(4):547-63.

Insert

Membrane-targeting sequence of Lyn kinase

Expression

Mammalian

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pLX-AS

Plasmid

#188870

Purpose

Compact T-DNA binary vector of the pLX series suitable for assembly of plant virus infectious clones and their Agrobacterium-mediated delivery to plants

Depositor

Fabio Pasin

Article

Pasin et al STAR Protoc. 2022 Dec 16;3(4):101716. do

Insert

none

Use

Synthetic Biology; Binary t-dna vector

Expression

Plant

Promoter

cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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HRE-UnaG

Plasmid

#201710

Purpose

Hypoxia responsive promoter driving UnaG fluorescent proteins

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Promoter

HRE-mCMV

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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HRE-dUnaG

Plasmid

#201711

Purpose

Hypoxia responsive promoter driving destablized UnaG fluorescent protein

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-Tn7-Pcym

Plasmid

#225280

Purpose

Mini-Tn7 pGP-Tn7-Cm vector with inducible Pcym cumate promoter for the inducible/controlled expression of target genes inserted into the attTn7 chromosomal site.

Depositor

Mark Schembri

Article

Chang et al Appl Environ Microbiol. 2024 Oct 23;90(1

Type

Empty backbone

Expression

Bacterial

Promoter

Pcym cumate (p-isopropylbenzoate)-inducible promo…

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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Flag Rab12 Q101L

Plasmid

#86788

Purpose

mammalian expression of Rab12 Q101L, constitutively GTP-bound mutant

Depositor

Peter McPherson

Article

Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

Insert

Rab12 (RAB12 Human)

Tags

Flag

Expression

Mammalian

Mutation

Q101L, constitutively GTP-bound mutant

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-Tn7-PrpsM

Plasmid

#225282

Purpose

Mini-Tn7 pGP-Tn7-Cm vector with constitutive PrpsM promoter for the constitutive expression of target genes inserted into the attTn7 chromosomal site.

Depositor

Mark Schembri

Article

Chang et al Appl Environ Microbiol. 2024 Oct 23;90(1

Type

Empty backbone

Expression

Bacterial

Promoter

Constitutive PrpsM promoter

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-Myo19

Plasmid

#127612

Purpose

GFP-tagged Myosin-19

Depositor

Josef Kittler

Article

Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

Insert

Myo19

Tags

eGFP

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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MAC_C_ROS1

Plasmid

#187766

Purpose

MAC-tagged gene expression of human ROS1

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

ROS1 (ROS1 Human)

Expression

Mammalian

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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