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pRSFDuet-sumo-h-cGAS

Plasmid

#127161

Purpose

expression of human cGAS protein

Depositor

Thomas Tuschl

Article

Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

Insert

MB21D1 (CGAS Human)

Use

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FGF2-K128N

Plasmid

#120284

Purpose

Produce human recombinant FGF2 K128N in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Basic fibroblast growth factor 2 (FGF2 Synthetic, Human)

Use

Tags

6x His

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1.sh.beta-catenin.1248

Plasmid

#19761

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

small hairpin RNA against beta-catenin (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pLKO.1.sh.beta-catenin.2279

Plasmid

#19762

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

small hairpin RNA against beta-catenin (CTNNB1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2009

Availability

Academic Institutions and Nonprofits only
pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Use

Tags

His6 and bdSUMO

Expression

Bacterial

Mutation

Promoter

T7 promoter and lac operator

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1aL-Cre-IRES-NeoR-W

Plasmid

#126700

Purpose

lentiviral or plasmid based co-expression of Cre recombinase and a neomycin resistance cassette

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

Cre-IRES

Use

Cre/Lox and Lentiviral

Tags

SV40 NLS

Expression

Mutation

Promoter

Ef1a

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLX313-TP53-P278A

Plasmid

#118015

Purpose

Constitutive expression of mutant human Tumor Protein p53 (TP53)

Depositor

David Root, William Hahn

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

TP53 (TP53 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

p.Pro278Ala

Promoter

EF1alpha

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR003-sgTP53-2

Plasmid

#118020

Purpose

Constitutive expression of sgRNA targeting human TP53

Depositor

David Root, William Hahn

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

sgTP53-2 (TP53 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR003-sgTP53-4

Plasmid

#118022

Purpose

Constitutive expression of sgRNA targeting human TP53

Depositor

David Root, William Hahn

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

sgTP53-4 (TP53 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR003-sgTP53-1

Plasmid

#118019

Purpose

Constitutive expression of sgRNA targeting human TP53

Depositor

David Root, William Hahn

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

sgTP53-1 (TP53 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-sumo-h-cGASCD(K427E/K428E)

Plasmid

#127162

Purpose

expression of truncated human cGAS protein

Depositor

Thomas Tuschl

Article

Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

Insert

MB21D1 (CGAS Human)

Use

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR003-sgTP53-3

Plasmid

#118021

Purpose

Constitutive expression of sgRNA targeting human TP53

Depositor

David Root, William Hahn

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

sgTP53-3 (TP53 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a-TALEN_HD (SPC right)

Plasmid

#126695

Purpose

right TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

human SPC right

Use

TALEN

Tags

NLS, truncated N term and TAL, FokI

Expression

Mammalian

Mutation

R451P in SPC

Promoter

EF1

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a-TALEN_NN (SPC left)

Plasmid

#126694

Purpose

left TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

human SPC left

Use

TALEN

Tags

0.5 repeat NN, TAL, FokI and NLS, truncated N ter…

Expression

Mammalian

Mutation

Promoter

EF1

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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p426-Cup1p-FUS-FusionRed

Plasmid

#188393

Purpose

Cu2+-dependent expression of N-terminal FUS fused with red fluorescent protein in yeast cells

Depositor

Michihiko Kataoka

Article

Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

Insert

FUS (FUS Human)

Use

Tags

FusionRed

Expression

Yeast

Mutation

Promoter

CUP1 promoter

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR003-sgTP53-5

Plasmid

#118023

Purpose

Constitutive expression of sgRNA targeting human TP53

Depositor

David Root, William Hahn

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

sgTP53-5 (TP53 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(BCL11B-E1(14))-PGKpuro2ABFP-W

Plasmid

#208554

Purpose

Lentiviral vector expressing gRNA targeting human BCL11B-E1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

BCL11B-E1(14) (BCL11B Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_His-TEV-EiCsm6

Plasmid

#172487

Purpose

Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

Enterococcus italicus Csm6 (codon optimized)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7 promoter and lac operator

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLe6Δ -hLP-mcs/(EGFP-IresPuro-hInt)

Plasmid

#64314

Purpose

chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells

Depositor

Noriyuki Tsumaki

Article

Outani et al PLoS One. 2013 Oct 16;8(10):e77365. doi:

Inserts

COL11A2 promoter (COL11A2 Human)
COL11A2 enhancer (COL11A2 Human)

Use

Lentiviral

Tags

EGFP and IRES-PURO

Expression

Mammalian

Mutation

N/A

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanEC1.45_min1-2_1XCoordinatorMutant

Plasmid

#173963

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 1 mutated Coordinator motif

Use

Luciferase

Tags

Expression

Mutation

1 mutated Coordinator motif

Promoter

SV40 promoter

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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