We narrowed to 15,797 results for: form

1. 8522-M31-663

   Plasmid

   #225661

   Purpose

   Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (red configuration)

   Depositor

   Dominic Esposito

   Article

   Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

   Insert

   dsCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SORE6-mCMVp

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pL-CRISPR.SFFV.tRFP

   Plasmid

   #57826

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   SFFV
   P2A-tRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. TRE3G-CTCF-dRBR-HA-MND-zeo

   Plasmid

   #232802

   Purpose

   Inducible over expression of RNA-binding domain deficient form of CTCF.

   Depositor

   Chunliang Li

   Article

   Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

   Insert

   Ctcf-dRBR (Ctcf Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE-puro TPR-Met

   Plasmid

   #10902

   Depositor

   Bob Weinberg

   Article

   Gupta et al Nat Genet. 2005 Oct . 37(10):1047-54.

   Insert

   TPR-Met (MET, TPR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Constitutively active form of the c-Met receptor

   Promoter

   Available since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. CROPseq-Guide-BSD

   Plasmid

   #216123

   Purpose

   Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the blasticidin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald

   Plasmid

   #225947

   Purpose

   Lentiviral vector plasmid expressing human CKAP4 mutations S3E S17E S19E under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva, Evan Reid

   Inserts

   CKAP4 (CKAP4 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   S3E S17E S19E

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-SFFV-CKAP4 S3A S17A S19A-WPRE-UbC-Emerald

   Plasmid

   #225948

   Purpose

   Lentiviral vector plasmid expressing human CKAP4 mutations S3A S17A S19A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva, Evan Reid

   Inserts

   CKAP4 (CKAP4 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   S3A S17A S19A

   Promoter

   SFFV and UbC

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQHA-USP11 CS puroR

   Plasmid

   #46750

   Purpose

   Retroviral vector that expresses catalytically inactive form of HA-tagged human USP11

   Depositor

   Gordon Peters, Goedele Maertens

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP11 (USP11 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C318S--catalytically inactive

   Promoter

   CMV

   Available since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFE-Reu-FI-prk

   Plasmid

   #162696

   Purpose

   Expresses R. eutropha Form IC rubisco and S. elongatus Prk

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   R. eutropha CbbLS and S. elongatus Prk
   

   Use

   Tags

   N terminal 6x His tag on PRK

   Expression

   Bacterial

   Mutation

   Promoter

   PLtet0-1 promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-SFFV-myc-RRBP1-WPRE-UbC-Emerald

    Plasmid

    #225942

    Purpose

    Lentiviral vector plasmid expressing human ribosome binding protein 1 (RRBP1) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva, Evan Reid

    Inserts

    RRBP1 (RRBP1 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGTM_COV2_NSP5_C145A_001_SUMO

    Plasmid

    #192349

    Purpose

    Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form

    Depositor

    Gaetano Montelione

    Article

    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

    Insert

    SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
    

    Use

    Tags

    6X-His-Sumo

    Expression

    Bacterial

    Mutation

    Cysteine 145 of the active site is substituted wi…

    Promoter

    T7 lac

    Available since

    June 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pEF1a_FL MUT TET1

    Plasmid

    #124081

    Purpose

    Expression of catalytic inactive form of full-length TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 - full length, mutated (TET1 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    H1672Y, D1674A

    Promoter

    EF1 alpha

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF1a_CD WT TET1

    Plasmid

    #124082

    Purpose

    Expression of wild-type catalytic domain only form of TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 catalytic domain (TET1 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1 alpha

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR KIF17 (1-738)

    Plasmid

    #60406

    Purpose

    Expression of truncated form of human homodimeric kinesin-2 (KIF17) amino acids 1-738

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    KIF17 (KIF17 Human)
    

    Use

    Lentiviral

    Tags

    GB1, Strep-Tag, and sfGFP

    Expression

    Mammalian

    Mutation

    Truncated version amino acids 1-738

    Promoter

    SFFV

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF1a_CD MUT TET1

    Plasmid

    #124083

    Purpose

    Expression of mutated catalytic domain only form of TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 - catalytic domain mutated (TET1 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    H1672Y, D1674A

    Promoter

    EF1 alpha

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pL-CRISPR.SFFV.PAC

    Plasmid

    #57829

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQFlag-USP11 CS puroR

    Plasmid

    #46748

    Purpose

    Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP11

    Depositor

    Gordon Peters, Goedele Maertens

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP11 (USP11 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C318S--catalytically inactive

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. NET5s pEGFP-N2 (1154)

    Plasmid

    #62034

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET5s (TMEM201 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    short form

    Promoter

    CMV

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

19. HOXA4 (murine) HA-tag pET

    Plasmid

    #8489

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXA4 (Hoxa4 Mouse)
    

    Use

    Tags

    HA and T7

    Expression

    Bacterial

    Mutation

    Thr-2 changed to Ala-2 to form better ribosomal b…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

20. Delta DED FLIP-L

    Plasmid

    #13790

    Depositor

    Guy Salvesen

    Article

    Boatright et al Biochem J. 2004 Sep 1. 382(Pt 2):651-7.

    Insert

    DELTA DED FLIP LONG (CFLAR Human)
    

    Use

    Tags

    8 x His tag

    Expression

    Bacterial

    Mutation

    Delta DED removes first 186 residues

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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