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pMYT018

Plasmid

#180671

Purpose

Encodes rtTA as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

rtTA

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT015

Plasmid

#180668

Purpose

Encodes pTet7 as a Type 2 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

pTet7

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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p{ie1-DsRed::GW,attB}

Plasmid

#182173

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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p{ie1-eGFP::GW,attB}

Plasmid

#182172

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

eGFP

Expression

Insect

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSpal2At

Plasmid

#78286

Purpose

Expresses A. thaliana PAL2 in E. coli

Depositor

David Nielsen

Article

McKenna et al Metab Eng. 2011 Sep;13(5):544-54. doi: 1

Insert

PAL2 (PAL2 Mustard Weed)

Expression

Bacterial

Mutation

*see below

Promoter

lac

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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p7XC3H

Plasmid

#47065

Purpose

FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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mEos4b-Caveolin-C-10

Plasmid

#57503

Purpose

Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Insert

Caveolin (CAV1 Human)

Tags

mEos4b

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pACYC.GFP1Amb

Plasmid

#102792

Purpose

Expresses GFP with a premature stop codon

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Insert

Green Fluorescent Protein

Expression

Bacterial

Mutation

Y39Stop

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAC-EsaR-EsaI

Plasmid

#47660

Purpose

esaI gene downstream of esaR in pAC-EsaR (CmR)

Depositor

Cynthia Collins

Article

Shong et al ACS Synth Biol. 2013 Jul 23.

Insert

esaI-term

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plac

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
AAV Synapsin Intron H2B Gcamp 6s wpre Pzac2.1

Plasmid

#74150

Purpose

calcium sensor

Depositor

Loren Looger

Insert

GCaMP6s

Use

AAV

Tags

H2B

Promoter

Synapsin

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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