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Addgene

We narrowed to 8,865 results for: BLI;

Showing: 4201 - 4250 of 8865 results
  1. pTRIPZ (M)-HT-Cbx2

    Plasmid
    #82510
    Purpose
    Expresses Halotag-Cbx2 fusion proteins in mammalian cells
    Depositor
    Insert
    Chromobox Homolog 2 (CBX2 Human)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    aggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaacc…
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3.1+ SMN1 myc HIS

    Plasmid
    #71687
    Purpose
    Mammalian expression of SMN1
    Depositor
    Insert
    SMN1 (SMN1 Human)
    Use
    Tags
    HIS and myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. ZNRF3-SmBiT, BiBiT-Ready Vector

    Plasmid
    #237083
    Purpose
    Express ZNRF3-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Has Service
    DNA
    Insert
    ZNRF3 (ZNRF3 Human)
    Use
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. pCDH-CB-iCre-P2A-tdTomato-T2A-Puro

    Plasmid
    #72255
    Purpose
    Express iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoter
    Depositor
    Insert
    iCre-P2A-tdTomato-T2A-Puro
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CB and chicken beta-actin + CMV enhancer
    Available since
    March 1, 2017
    Availability
    Academic Institutions and Nonprofits only
  5. NPY-GFP

    Plasmid
    #74629
    Purpose
    human neuropeptide Y fused to GFP
    Depositor
    Insert
    Neuropeptide Y (NPY Human)
    Use
    Tags
    mEGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2016
    Availability
    Academic Institutions and Nonprofits only
  6. pLV V5-lamin B1

    Plasmid
    #175108
    Purpose
    Lentiviral expression of V5-tagged mouse Lmnb1
    Depositor
    Insert
    Lmnb1 (Lmnb1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pDEST26-PPP2CB-C-HA

    Plasmid
    #195179
    Purpose
    Vector for transient expression of PPP2CB gene with C-terminal HA tag, gene flanked by att sites for gateway cloning
    Depositor
    Insert
    PPP2CB (PPP2CB Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. pRK5-EGFR

    Plasmid
    #65225
    Purpose
    Expression of EGFR in mammalian cells
    Depositor
    Insert
    EGFR (EGFR Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
  9. pENTR1A-Huwe1

    Plasmid
    #37431
    Depositor
    Insert
    Huwe1 (HUWE1 Human)
    Use
    Gateway entry
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 25, 2012
    Availability
    Academic Institutions and Nonprofits only
  10. FVIII H2

    Plasmid
    #46773
    Purpose
    pcDNA4.full length FVIII D1241E
    Depositor
    Insert
    full length FVIII D1241E (F8 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    D1241E
    Promoter
    CMV
    Available since
    Nov. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
  11. pLVXPuro-mGFP-LIC2

    Plasmid
    #66601
    Purpose
    Expresses Dynein-1 LIC2 as a mGFP fusion
    Depositor
    Insert
    LIC2 (DYNC1LI2 Human)
    Use
    Lentiviral
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 28, 2015
    Availability
    Academic Institutions and Nonprofits only
  12. YIp204-PADH1-atTIR1-9myc

    Plasmid
    #99532
    Purpose
    S. cerevisiae expression of F-box protein TIR1-9myc under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)
    Depositor
    Insert
    TIR1 (TIR1 Mustard Weed)
    Use
    Tags
    9xMyc
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
  13. pLenti-NPY-mNeonGreen

    Plasmid
    #232456
    Purpose
    Expresses human NPY-mNeonGreen fusion in mammalian cells
    Depositor
    Insert
    NPY-mNeonGreen (NPY Human)
    Use
    Lentiviral
    Tags
    mNeonGreen
    Expression
    Mutation
    Promoter
    EF1 alpha
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. pEF1a-PEmax-P2A-GFP

    Plasmid
    #202000
    Purpose
    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.
    Depositor
    Insert
    PEmax-P2A-GFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
  15. Myc-Foxo1 AAA (pCMV5)

    Plasmid
    #17547
    Depositor
    Insert
    Foxo1 AAA (Foxo1 Mouse)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Changed T24A T253A S316A
    Promoter
    CMV
    Available since
    Oct. 20, 2008
    Availability
    Academic Institutions and Nonprofits only
  16. pGEX 6P1 CDK2 (552)

    Plasmid
    #61845
    Purpose
    bacterial expression of GST CDK2 WT
    Depositor
    Insert
    CDK2 (CDK2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
  17. Mek1

    Plasmid
    #40774
    Depositor
    Insert
    MKK1 (MAP2K1 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Starting Met removed
    Promoter
    CMV
    Available since
    Oct. 15, 2012
    Availability
    Academic Institutions and Nonprofits only
  18. POLG-pcDNA3.1

    Plasmid
    #191487
    Purpose
    Expresses human POLG gene on pcDNA3.1 backbone. POLG gene is cloned with BamHI and XbaI restriction sites.
    Depositor
    Insert
    POLG (POLG Human)
    Use
    Tags
    V5 tag, 6X His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  19. pKG123

    Plasmid
    #63074
    Purpose
    Retroviral expression of mCherry-TEV-S-tag CENP-C for protein localization and affinity purification (LAP)
    Depositor
    Insert
    CENP-C (CENPC Human)
    Use
    Retroviral
    Tags
    TEV-S and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2016
    Availability
    Academic Institutions and Nonprofits only
  20. pcDNA3.1 CLN3-GFP

    Plasmid
    #78110
    Purpose
    Mammalian expression of human CLN3 with C terminal GFP fusion
    Depositor
    Insert
    CLN3 (CLN3 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2016
    Availability
    Academic Institutions and Nonprofits only
  21. pQdCas12a.sggfp(C)

    Plasmid
    #236042
    Purpose
    The plasmid pQdCas12a.sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene.
    Insert
    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
  22. pLV-Enh eGFP Reporter-muKC2-X11

    Plasmid
    #59235
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Insert
    Krt7-ex2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
  23. pCI-ING3

    Plasmid
    #79070
    Purpose
    Expression of p47 ING3 in mammalian cells
    Depositor
    Insert
    ING3 (p47 ING3 isoform) (ING3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
  24. mEmerald-ALK5-N-13

    Plasmid
    #62751
    Purpose
    Localization: Membrane Receptor, N1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Insert
    ALK5 (TGFBR1 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
  25. pDONOR_MM001-ZFP143-FKBPV-2HA-P2A-GFP-targetting construct

    Plasmid
    #212705
    Purpose
    targeting vector to introduce an FKBPV-2HA-P2A-GFP sequence at the C terminal of Zfp143 locus . Degradation tag (dTAG) system
    Depositor
    Insert
    ZFP143-FKBPV-2HA-P2A-GFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  26. pFastBac1_FLAG-BPTF

    Plasmid
    #102242
    Purpose
    Expression of human Flag-BPTF using baculovirus from pFastBac1
    Depositor
    Insert
    Flag-BPTF (BPTF Human)
    Use
    Tags
    Flag
    Expression
    Insect
    Mutation
    Promoter
    Polyhedron
    Available since
    Dec. 11, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
  27. pTF272 (pFA6a-TEV-6xGly-3xFlag-HphMX)

    Plasmid
    #44083
    Depositor
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Expression
    Mutation
    Promoter
    Available since
    April 3, 2013
    Availability
    Academic Institutions and Nonprofits only
  28. EF1α-AausFP1-Z1

    Plasmid
    #135614
    Purpose
    AausFP1 - bright green fluorescent protein expression from A. australis under EF1a-HTLV promoter in minimal backbone plasmid.
    Depositor
    Insert
    AausFP1
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a-HTLV
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
  29. pLV-EF1a-V5-Bet1

    Plasmid
    #120243
    Purpose
    Expresses V5-tagged Bet1 in lentiviral vector
    Depositor
    Insert
    Bet1 (Bet1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  30. pTF268 (pFA6a-TEV-6xGly-3xFlag-KanMX)

    Plasmid
    #44095
    Depositor
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    3xFlag, 6xGly linker, ADH1 transcription terminat…
    Expression
    Mutation
    Promoter
    Available since
    April 10, 2013
    Availability
    Academic Institutions and Nonprofits only
  31. mEos4b-CenpA-C-22

    Plasmid
    #62775
    Purpose
    Localization: Nucleus, C1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581
    Insert
    CenpA (CENPA Human)
    Use
    Tags
    mEos4b
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
  32. pEC1.2/EC1.1_Cas9i_TagRFP

    Plasmid
    #227249
    Purpose
    CRISPR plasmid with Cas9i expressed under egg cell promoter - TagRFP seed selection
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    pEC1.2/EC1.1
    Available since
    Nov. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
  33. Pet28a_Synthetic_Human_H2AZ.1

    Plasmid
    #42629
    Depositor
    Insert
    Human H2AZ.1 (H2AZ1 Codon Optimized, Human, Synthetic)
    Use
    Tags
    Untagged
    Expression
    Bacterial
    Mutation
    Codon Optimized
    Promoter
    T7
    Available since
    March 21, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
  34. pLV-Enh eGFP Reporter-muKC2-X1

    Plasmid
    #59200
    Purpose
    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.
    Depositor
    Insert
    krt1-ex1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Mu Hsp68 minimal promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
  35. HaloTag-G protein gamma-2

    Plasmid
    #67018
    Purpose
    G protein gamma-2 tagged with HaloTag on amino terminus
    Depositor
    Insert
    HaloTag-G protein gamma-2 (GNG2 Human)
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  36. pEGFP Cited2

    Plasmid
    #112246
    Purpose
    expression of EGFP-Cited2 in mammalian cells
    Depositor
    Insert
    Cited2 (CITED2 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
  37. pcDNA3.1-Flag-RBM24

    Plasmid
    #208052
    Purpose
    Expressing N-ter Flag tagged human RBM24 (ENST00000379052) in mammalian cells
    Depositor
    Insert
    human RBM24 (ENST00000379052) coding region (RBM24 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  38. pAAV-DIO-CAG-hM3Dq-P2A-dTomato

    Plasmid
    #202036
    Purpose
    AAV-mediated, cre-dependent expression of the pharmacogenetic actuator hM3Dq-2A-dTomato
    Insert
    hM3Dq
    Use
    AAV
    Tags
    dTomato
    Expression
    Mutation
    Promoter
    CAG
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
  39. pBSJL1

    Plasmid
    #71261
    Purpose
    E. coli-Veillonella shuttle plasmid
    Depositor
    Type
    Empty backbone
    Use
    E. coli-veillonella shuttle plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
  40. pCMV-ABE-P48R

    Plasmid
    #161816
    Purpose
    Expresses ABE-P48R in mammalian cells
    Depositor
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
  41. pcDNA3.3_SARS2_XBB.1.16

    Plasmid
    #201189
    Purpose
    expressing SARS-CoV-2 XBB.1.16 spike protein for pseudovirus production
    Depositor
    Insert
    Spike (XBB.1.16) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E180V, T(K)478R on the basis of XBB.1.5
    Promoter
    CMV
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
  42. HaloTag-LacI #1691

    Plasmid
    #232636
    Purpose
    lac repressor (LacI) which binds lacO sites; labeled with HaloTag
    Depositor
    Insert
    HaloTag-LacI
    Use
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
  43. Banshee RunxDN mCherry

    Plasmid
    #80158
    Purpose
    retroviral expression of Runx1DN and mCherry
    Depositor
    Insert
    Runx1 (Runx1 Mouse)
    Use
    Retroviral
    Tags
    HA and IRES-mCherry
    Expression
    Mammalian
    Mutation
    DN--truncated version of the Runx1 protein contai…
    Promoter
    Available since
    July 26, 2016
    Availability
    Academic Institutions and Nonprofits only
  44. pTRIPZ(M)-HT-Cbx8

    Plasmid
    #82516
    Purpose
    Lentivirus vector. Expresses halotag Cbx8fusion proteins in mammalian cells.
    Depositor
    Insert
    Chromobox Homolog 8 (CBX8 Human)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    cmv
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
  45. pLJM1-4EBP1-T37A/T46A

    Plasmid
    #136420
    Purpose
    Stable overexpression of elF4EBP1 T37A/T46A
    Depositor
    Insert
    eIF4EBP1 (EIF4EBP1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    T37A/T46A
    Promoter
    CMV
    Available since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
  46. pUAST_mLExA-VP16-NFATc1 (C-del)

    Plasmid
    #35023
    Depositor
    Insert
    NFATc1 (NFATC1 Human)
    Use
    Tags
    VP16 and mLexA
    Expression
    Insect
    Mutation
    deletion of amino acids 590-716
    Promoter
    UAS
    Available since
    March 8, 2012
    Availability
    Academic Institutions and Nonprofits only
  47. pT7-V5-SBP-C1-HshnRNPC1

    Plasmid
    #64920
    Purpose
    Expresses V5- and SBP-tagged hnRNPC1 in mammalian cells
    Depositor
    Insert
    hnRNPC1 (HNRNPC Human)
    Use
    Tags
    V5 and SBP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
  48. hKv2.1-pEGFP-C1

    Plasmid
    #111538
    Purpose
    Expresses human KCNB1 in mammalian cells for imaging
    Depositor
    Insert
    human Kv2.1 (KCNB1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
  49. NET43 pEGFP-N2 (609)

    Plasmid
    #61991
    Purpose
    mammalian expression of nuclear envelope transmembrane protein
    Depositor
    Insert
    NET43 (NAT10 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 4201 - 4250 of 8865 results