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We narrowed to 14,061 results for: CAN

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Showing: 1681 - 1700 of 14061 results

  8. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid
    #181781
    Purpose
    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    Expression
    Bacterial
    Mutation
    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181781-plasmid-map-sequence-id-354678

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  16. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

    Plasmid
    #181791
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181791-plasmid-map-sequence-id-354814

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  17. pLC133

    Plasmid
    #198639
    Purpose
    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.
    Depositor
    David Bikard
    Article
    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.
    Insert
    dCas9
    Tags
    3x-FLAG
    Expression
    Bacterial
    Promoter
    pTet
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198639-plasmid-map-sequence-id-390787

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  19. pCIFR-3

    Plasmid
    #233295
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    msfGFP
    Promoter
    p14G
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233295-plasmid-map-sequence-id-468475

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  20. pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)

    Plasmid
    #181789
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTniQ, ShCas12k-ShTnsC
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181789-plasmid-map-sequence-id-354816

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Showing: 1681 - 1700 of 14061 results