We narrowed to 10,971 results for: NSI;

1. Lck-cpmTq2-Calcium-lifetime-sensor

   Plasmid

   #129627

   Purpose

   Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   Lck-cpmTq2-Calcium-lifetime-sensor
   

   Use

   Tags

   Lck

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-4eCOL2A1

   Plasmid

   #97211

   Purpose

   For cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectors

   Depositor

   Ryan Porter

   Article

   De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

   Insert

   4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
   

   Use

   Vector is designed for cloning and generation of …

   Tags

   Expression

   Mutation

   Promoter

   COL2A1 regulatory elements

   Available since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSB137 - pL2_pSB90_pNOS::rLUC-I::tNOS_tOCS::fLUC-I::pSTR1

   Plasmid

   #123194

   Purpose

   binary plant vector for transient dual luciferase (rLUC & fLUC with introns) expression

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   pNOS::rLUC-I::tNOS tOCS::fLUC-I::pSTR1
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHub10-His-YFP-PIP2;1

   Plasmid

   #99386

   Purpose

   overexpression and isolation of recombinant At-PIP2;1 protein

   Depositor

   Ning Li

   Article

   Qing et al Mol Plant. 2016 Jan 4;9(1):158-74. doi:

   Insert

   PIP2;1 (PIP2A Mustard Weed)
   

   Use

   Tags

   His and YFP

   Expression

   Plant

   Mutation

   Promoter

   2xCaMV35S

   Available since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xnls-cpmTq2-Calcium-lifetime-sensor

   Plasmid

   #129626

   Purpose

   Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes

   Depositor

   Dorus Gadella

   Article

   van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

   Insert

   3xnls-cpmTq2-Calcium-lifetime-sensor
   

   Use

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)

   Plasmid

   #153442

   Purpose

   E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.XDocB
   

   Use

   Tags

   3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprin…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA4TO R99A CoV-2 nsp1 3xFLAG

   Plasmid

   #176061

   Purpose

   Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

   Depositor

   Britt Glaunsinger

   Article

   Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

   Insert

   CoV-2 nsp1 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe-PI(WT)-Rac1(WT)-mCherry

   Plasmid

   #87550

   Purpose

   PI(WT): Photo-inhibitable/blue light sensitive Rac1

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   Rac1 (RAC1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe-PI(WT)-Rac1(Q61L)-mCherry

   Plasmid

   #87553

   Purpose

   PI(WT): Photo-inhibitable/blue light sensitive; const. active Rac1

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   Rac1 (RAC1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Q61L

   Promoter

   CMV

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA4TO-3XFLAG Halo delta 1-117 nsp1

    Plasmid

    #175423

    Purpose

    Expresses delta 1-117 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 delta 1-117 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    nsp1 C-terminus

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA4 3xFLAG Halo delta 118-180 nsp1

    Plasmid

    #175424

    Purpose

    Expresses delta 118-180 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 delta 118-180 nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminus of nsp1

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA4TO-3XFLAG Halo R124A/K125A nsp1

    Plasmid

    #175417

    Purpose

    Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 R124A/K125A nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    R124A and K125A

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p-AAV2-hSyn-MerMAID6-Citrine

    Plasmid

    #126521

    Purpose

    Anion-conducting Channelrhodopsin with near-complete desensitization in continuous light. Codon-optimized for mammalian expression (human/mouse).

    Depositor

    Peter Hegemann

    Article

    Oppermann et al Nat Commun. 2019 Jul 25;10(1):3315. doi:

    Insert

    MerMAID6
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mutation

    Promoter

    human synapsin

    Available since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCSP1-mp53AS-6xMUT

    Plasmid

    #20904

    Depositor

    Stefano Piccolo

    Article

    Cordenonsi et al Science. 2007 Feb 9. 315(5813):840-3.

    Insert

    p53AS (Trp53 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    point mutation (Ser/Thr-Ala) of the 6 N-terminal …

    Promoter

    Available since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pCDF_AtRaf1/Raf2/BSD2

    Plasmid

    #229511

    Purpose

    Expresses Arabidopsis thaliana chaperones: Raf1,Raf2,BSD2

    Depositor

    Laura Gunn

    Article

    Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

    Inserts

    Rubisco accumulation factor 1 (AT3G04550 Mustard Weed)
    Rubisco accumulation factor 2 (AT5G51110 Mustard Weed)
    BSD2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N terminus chloroplast transit peptide truncation

    Promoter

    T7

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG, I253A, H310A, H435A

    Plasmid

    #222853

    Purpose

    Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc lacking FcRn binding

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Use

    Tags

    IgG1_Fc

    Expression

    Mammalian

    Mutation

    L234A, L235A, P329G, H435A, I253A, H310A

    Promoter

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. hTln1-R12DD-SCLS

    Plasmid

    #191443

    Purpose

    expresses a variant of human talin1 domains R12-DD, with a deletion between K2375 and A2397

    Depositor

    Ben Goult

    Article

    Elefant et al JCI Insight. 2024 Dec 20;9(24):e173664.

    Insert

    Human Talin1 R12DD (TLN1 Human)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    deletion between residues K2375 and A2397

    Promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4TO-SCN8A-Variant-3-IRES-mScarlet

    Plasmid

    #209411

    Purpose

    Expression of wild-type adult isoform of human SCN8A.

    Depositor

    Al George

    Article

    Vanoye et al JCI Insight. 2024 May 21;9(12):e177530.

    Insert

    SCN8A Variant 3, stabilized (SCN8A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Modifed human HSPA5 intron 4 inserted after bp237…

    Promoter

    CMV

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA4 3xFLAG Halo G-Linker nsp1

    Plasmid

    #175426

    Purpose

    Expresses G-linker CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    G-linker nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    G-linker in place of the RNase destabilization do…

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA4 3xFLAG Halo R119A K120A nsp1

    Plasmid

    #175428

    Purpose

    Expresses R119A K120A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    nsp1 R119A K120A
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    N-terminal mutants R119A K120A

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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