We narrowed to 24,400 results for: lars

1. WDV-Echistatin

   Plasmid

   #226985

   Purpose

   Expresses the HUH endonuclease WDV fused at the C termini with Echistatin to target RGD binding integrins with DNA tension sensors

   Depositor

   Wendy Gordon

   Article

   Pawlak et al Nat Commun. 2023 Apr 28;14(1):2468. doi:

   Inserts

   WDV (repA )
   Echistatin
   

   Use

   Tags

   6xHis and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCWXPGR-pTF-betaCatenin

   Plasmid

   #114281

   Purpose

   TET-inducible expression of a constitutively active form of the BetaCatenin protein

   Depositor

   Patrick Salmon

   Article

   Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

   Insert

   Catenin beta 1 (CTNNB1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   deletion of AA 1 to 45

   Promoter

   Available since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-cFos-XRI-V5

   Plasmid

   #178058

   Purpose

   Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with V5 tag, under c-fos promoter.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   XRI-V5
   

   Use

   AAV

   Tags

   V5-MBP

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse c-fos promoter

   Available since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM1 flag-cFLIP

   Plasmid

   #211529

   Purpose

   Expresses flag-tagged cFLIP (CFLAR)

   Depositor

   Richard Possemato

   Article

   Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

   Insert

   cFLIP (CFLAR Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. MultiMate-Rainbow

   Plasmid

   #206252

   Purpose

   Expression of 7 fluorescently labelled proteins in mammalian cells. Can be used to generate recombinant baculovirus particles.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   H2B (human); Actin, Tubulin (B. taurus); mito mCherry, GST mTagBFP1 (Synthetic); CyOFP1 (E. quadricolor); Ctnnb1 (mouse)
   

   Use

   Recombinant baculovirus production (bac-to-bac)

   Tags

   iRFP713

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-sgCD71-2

   Plasmid

   #46918

   Purpose

   Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CD71 gene

   Depositor

   Jonathan Weissman, Stanley Qi

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Inserts

   sgCD71 -2 (TFRC Human)
   Puromycin resistance and mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. MultiMate-Cell-cycle

   Plasmid

   #206280

   Purpose

   Expression of a FUCCI cell cycle sensor with a H2B iRFP713 chromatin reporter. Can be used to generate recombinant baculovirus particles.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   H2B iRFP713, mAG-hGeminin, mKO2-hCdt1
   

   Use

   Recombinant baculovirus production

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-UBC-XRI-HA

   Plasmid

   #178056

   Purpose

   Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.

   Depositor

   Edward Boyden

   Article

   Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   XRI-HA
   

   Use

   AAV

   Tags

   HA-MBP

   Expression

   Mammalian

   Mutation

   Promoter

   Human ubiquitin C (UBC) promoter

   Available since

   Oct. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-LTR-dCas9-p65AD-BFP

   Plasmid

   #46913

   Purpose

   Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP

   Depositor

   Jonathan Weissman, Stanley Qi

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Inserts

   dCas9-p65AD-BFP fusion
   Puromycin resistance
   

   Use

   CRISPR and Retroviral

   Tags

   2xNLS, BFP, and VP64 domain

   Expression

   Mammalian

   Mutation

   Promoter

   LTR and PGK

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. FKBP-beta2(616-951)-GFP

    Plasmid

    #100726

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Long isoform

    Promoter

    CMV

    Available since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMS2284

    Plasmid

    #158419

    Purpose

    AAV plasmid with Ple349 (PDE6H MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple349 (PDE6H Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple349

    Available since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEMS2214

    Plasmid

    #158417

    Purpose

    AAV plasmid with Ple265 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple265 (PCP2 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple265

    Available since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMMK ENTR 1 CMV EYFP Tubulin

    Plasmid

    #206254

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes EYFP Tubulin under the control of CMV promoter.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    EYFP Tubulin
    

    Use

    Multimate/gateway entr 1

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMMK ENTR 2 CMV mTFP1 Actin

    Plasmid

    #206255

    Purpose

    ENTR Vector 2 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTFP1 Actin under the control of CMV promoter.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    mTFP1 Actin
    

    Use

    Multimate/gateway entr 2

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMMK ENTR 3 CMV mito mCherry

    Plasmid

    #206256

    Purpose

    ENTR Vector 3 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mitochondrially localised mCherry under the control of CMV promoter.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    mito mCherry
    

    Use

    Multimate/gateway entr 3

    Tags

    COX8 mitochondrial tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. FKBP-beta3(702-1094)-GFP

    Plasmid

    #100734

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP3B1 (AP3B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    appendage and ear only

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. iSH-iRFP670-sspB

    Plasmid

    #176102

    Purpose

    Heterodimerization with iLID, phosphatidylinositol 3-kinase derived iSH

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    phosphatidylinositol 3-kinase (Pik3r1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEMS2271

    Plasmid

    #158418

    Purpose

    AAV plasmid with Ple341 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple341 (PCP2 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple341

    Available since

    Feb. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. FKBP-beta2(616-951)-mRuby2

    Plasmid

    #100730

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    FKBP and mRuby2

    Expression

    Mammalian

    Mutation

    Long isoform

    Promoter

    CMV

    Available since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. CD8-pTagRFP657-LOVpep (T406A, T407A, I532A)

    Plasmid

    #100742

    Purpose

    To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CD8A (CD8A Human)
    

    Use

    Tags

    LOVpep and tagRFP657

    Expression

    Mammalian

    Mutation

    Truncated

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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