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We narrowed to 2,995 results for: mos

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Showing: 1681 - 1700 of 2995 results

  6. pZC08 (pFA6a-PSc-6xGly-PrtA-HphMX)

    Plasmid
    #44078
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, HphM…
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44078-plasmid-map-sequence-id-61691

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  7. pZC06 (pFA6a-PSc-6xGly-PrtA-KanMX)

    Plasmid
    #44090
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, KanM…
    Available since
    April 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    44090-plasmid-map-sequence-id-61689

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  10. pB80-SSPB(micro)-GFP-ppKin14VIb(861-1321)

    Plasmid
    #174639
    Purpose
    Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)
    Depositor
    Lukas Kapitein
    Publication
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SSPB(micro)-GFP-ppKinVIb(861-1321)
    Tags
    SSPB(micro)-GFP
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174639-plasmid-map-sequence-id-346670

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  11. MADR pDonor smFP-Myc (dark) WPRE

    Plasmid
    #130987
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-Myc, a cytoplasmic fluorescent protein which includes multiple Myc epitope tags
    Depositor
    Joshua Breunig
    Publication
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-Myc WPRE
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    Myc - 10 total Myc tags
    Mutation
    "dark" variant with GGG fluorophore ver…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    130987-plasmid-map-sequence-id-305000

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  13. pUb-Cas9-EGFP_cU6:6-sgRNA

    Plasmid
    #190597
    Purpose
    The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.
    Depositor
    Claudia Rueckert
    Publication
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    EGFP - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190597-plasmid-map-sequence-id-379952

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  14. pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN- Incenp-GFP

    Plasmid
    #108484
    Purpose
    inducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFP
    Depositor
    Susanne Lens
    Publication
    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CenpB-dCEN-INCENP (INCENP Human)
    Tags
    EGFP and VSV
    Expression
    Mammalian
    Mutation
    CEN box replaced by CenpB 1-166
    Promoter
    CMV
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    108484-plasmid-map-sequence-id-208768

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  15. pDual-eGFP(Y203)

    Plasmid
    #63559
    Purpose
    Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Publication
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Y203)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed threonine at position 203 with respect to…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)

    Plasmid
    #174642
    Purpose
    Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport
    Depositor
    Lukas Kapitein
    Publication
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)
    Tags
    SSPB(micro)-Venus
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    174642-plasmid-map-sequence-id-346995

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Showing: 1681 - 1700 of 2995 results