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We narrowed to 1,943 results for: rigi

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Showing: 1681 - 1700 of 1943 results

  1. pBIRC5-Split PS Intein-N tTA

    Plasmid
    #242033
    Purpose
    N-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the BIRC5 promoter.
    Depositor
    Vladislav Verkhusha
    Article
    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:
    Insert
    Split PS Intein-N tTA
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    The CMV promoter was replaced with the BIRC5 prom…
    Promoter
    hTERT
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242033-plasmid-map-sequence-id-483131

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  2. phTERT-Split PS Intein-N tTA

    Plasmid
    #242034
    Purpose
    N-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the hTERT promoter.
    Depositor
    Vladislav Verkhusha
    Article
    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:
    Insert
    Split PS Intein-N tTA
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    The CMV promoter was replaced with the hTERT prom…
    Promoter
    hTERT
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242034-plasmid-map-sequence-id-481812

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  3. pREC1010C-Eco1

    Plasmid
    #240690
    Purpose
    RSF1010 origin of replication plasmid containing Eco1 recombitron with a SapI flanked stuffer in the ncRNA expressed by J23115 constitutive promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Eco1 RT, Eco1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240690-plasmid-map-sequence-id-483599

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  4. pCR197

    Plasmid
    #240643
    Purpose
    Expression of the tri-cistronic construct NES-AmNeonGreen-P2A-mTurquoise2-NLS-T2A-mScarlet-I-PTS1 in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymerase
    Depositor
    John Pringle
    Inserts
    AIPGENE865 promoter
    SP6 promoter
    AmNeonGreen
    mTurquoise2
    mScarlet-I
    Use
    Gene expression and genomic integration in fish
    Tags
    NES (from Exaiptasia diaphana MEK2), NLS (from SV…
    Mutation
    Codon-optimized for Exaiptasia diaphana, amino ac…
    Available Since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    240643-plasmid-map-sequence-id-482910

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  5. pREC1010-opEbu1

    Plasmid
    #240702
    Purpose
    RSF1010 origin of replication plasmid containing Ebu1 recombitron with extended a1 a2 regions with a donor in the ncRNA targeting lacZ locus in Citrobacter freundii ATCC 8090 expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Ebu1 RT, Ebu1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    Extended a1 a2 regions
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240702-plasmid-map-sequence-id-482374

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  6. pREC1010-Abe1

    Plasmid
    #240683
    Purpose
    RSF1010 origin of replication plasmid containing Abe1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Abe1 RT, Abe1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240683-plasmid-map-sequence-id-482346

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  11. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2

    Plasmid
    #201533
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Dan Park
    Article
    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d
    Insert
    ilvA-relE_RBS3_CB2
    Use
    Synthetic Biology
    Mutation
    rhaR L128+1bp (frameshift), ilvA G455C
    Promoter
    PrhaBAD (rhamnose)
    Available Since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201533-plasmid-map-sequence-id-398133

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  12. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4

    Plasmid
    #201535
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Dan Park
    Article
    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d
    Insert
    ilvA-relE_RBS3_CB4
    Use
    Synthetic Biology
    Mutation
    rhaS ΔM1-H5, PrhaSR Δ179bp
    Promoter
    PrhaBAD (rhamnose)
    Available Since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201535-plasmid-map-sequence-id-398128

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  13. pRA1SEGFPTcIfm

    Plasmid
    #193777
    Purpose
    Cassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    EGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PR promoter and PLTetO-1
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193777-plasmid-map-sequence-id-389829

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  14. pTA1cI-T3

    Plasmid
    #193789
    Purpose
    Expresses truncated version of lambda CI named T3 under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    Truncated version of Lambda CI, named T3, where the first two codons are deleted
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PLTetO-1
    Available Since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193789-plasmid-map-sequence-id-389812

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  15. Venus-Bik-del72-136-pEGFP-C3

    Plasmid
    #191411
    Purpose
    Transfection of this plasmid will express "VBik-d72-136" (Venus fused to the N-terminus of the BH3-only protein, Bik that has amino acids 72-136 deleted).
    Depositor
    David Andrews
    Insert
    Bcl-2-interacting killer (BIK Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    with deletion of amino acids 72-136 (del72-136) i…
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    191411-plasmid-map-sequence-id-376884

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  16. THI ONLY pALD4 T-

    Plasmid
    #161497
    Purpose
    Non activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2 (non activation control NAC)
    gRNA (Non targeting control NTC)
    Expression
    Yeast
    Promoter
    pALD4, pPOR1, and pTEF2
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161497-plasmid-map-sequence-id-326273

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  18. pSTAP-seq_fly-zfh1_spikeIn-1

    Plasmid
    #86387
    Purpose
    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer & D. pseudoobscura eve core promoter
    Use
    Stap-seq spike in vector
    Available Since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    86387-plasmid-map-sequence-id-193668

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  19. pSTAP-seq_fly-zfh1_spikeIn-3

    Plasmid
    #86389
    Purpose
    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#1
    Use
    Stap-seq spike in vector
    Available Since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86389-plasmid-map-sequence-id-173956

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  20. pSTAP-seq_fly-zfh1_spikeIn-4

    Plasmid
    #86390
    Purpose
    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter
    Depositor
    Alexander Stark
    Article
    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103
    Insert
    D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#2
    Use
    Stap-seq spike in vector
    Available Since
    March 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86390-plasmid-map-sequence-id-173960

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Showing: 1681 - 1700 of 1943 results