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N-term Hec1-targeted Aurora B FRET sensor

Plasmid

#45239

Purpose

sensor targeted to N-terminus of Hec1

Depositor

Michael Lampson

Article

Welburn et al Mol Cell. 2010 May 14;38(3):383-92. doi:

Insert

Hec1, Aurora B FRET sensor

Use

Tags

Aurora B FRET sensor and Hec1

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pCI-neo:alpha4-mcherry

Plasmid

#45073

Depositor

Henry Lester

Article

Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

Insert

alpha4-mcherry (Chrna4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-MCPIP1(150-453)

Plasmid

#90109

Purpose

Mammalian expression of Flag-MCPIP1(150-453)

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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YFP(1-158) in pcDNAI/Amp

Plasmid

#60888

Purpose

This vector attaches YFP(1-158) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

Insert

YFP(1-158)

Use

Tags

Expression

Mammalian

Mutation

Met was substituted for Gln-69.

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCLC3sFlag

Plasmid

#52422

Purpose

mammalian expression of flag tagged rat CLC3 short transcript

Depositor

Steven Weinman

Article

Shimada et al Am J Physiol Gastrointest Liver Physiol.

Insert

CLC3 (Clcn3 Rat)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-cofilin1(S3A)-P2A-mTurquoise2

Plasmid

#163610

Purpose

Expresses constitutively active cofilin and mTurquoise2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

cofilin1 (Cfl1 Mouse)

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-SIRT3

Plasmid

#66167

Purpose

Expresses SIRT3 via retroviral method

Depositor

Ronald Kahn

Article

Jing et al Diabetes. 2013 Oct;62(10):3404-17. doi:

Insert

SIRT3 (Sirt3 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

L235P

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only
cmamxA1-GFP

Plasmid

#107195

Purpose

Expresses porcine annexin A1-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted

Depositor

Volker Gerke, Ursula Rescher

Article

Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

Insert

cmannexin A1

Use

Tags

Green Fluorescent Protein

Expression

Mammalian

Mutation

exchanges Asp170Ala, Glu255Ala, Glu330Ala, all ty…

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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YFP(159-238) in pcDNAI/Amp

Plasmid

#60889

Purpose

This vector attaches YFP(159-238) to the N-terminus of a protein.

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8

Insert

YFP(159-238)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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plko_TRE3G_Myc-Sox9HMGonlySTOP_PGKpuroR

Plasmid

#203963

Purpose

Dox inducible myc tag truncated SOX9 without trans-activating domain (noTA)

Depositor

Elaine Fuchs

Article

Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

Insert

SOX9-noTA (Sox9 Mouse)

Use

Lentiviral

Tags

myc

Expression

Mutation

the TA domain is truncated

Promoter

TRE3G

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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plko_TRE3G_nlsMyc-Sox9HMGtruncation_PGKpuroR

Plasmid

#203964

Purpose

Dox inducible myc tag truncated SOX9 without DNA-binding domain (noHMG)

Depositor

Elaine Fuchs

Article

Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

Insert

SOX9-noHMG (Sox9 Mouse)

Use

Lentiviral

Tags

myc

Expression

Mutation

the HMG domain is truncated

Promoter

TRE3G

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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hnRNPAB X2-RBD

Plasmid

#216516

Purpose

Expresses MBP-tagged RNA binding domain of hnRNPAB X2

Depositor

Nicolas Fawzi

Article

O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

Insert

hnRNPAB X2-RBD

Use

Tags

6xHis tags-MBP

Expression

Bacterial

Mutation

deleted amino acids 2-64 and 226-304

Promoter

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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hnRNPAB X2-IDR

Plasmid

#216517

Purpose

Expresses MBP-tagged intrinsically disordered region of hnRNPAB X2

Depositor

Nicolas Fawzi

Article

O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

Insert

hnRNPAB X2-IDR

Use

Tags

6xHis tags-MBP

Expression

Bacterial

Mutation

deleted amino acids 2-225

Promoter

Available since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDIV299

Plasmid

#204967

Purpose

AMA1 plasmid with Aspergillus optimized Mad7 and argB selection marker

Depositor

Uffe Mortensen

Article

Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

Inserts

Mad7
argB

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Mutation

Promoter

Aspergillus nidulans tef1 promoter and native pro…

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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Pt NaV1.4

Plasmid

#200233

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Phyllobates terriblis NaV1.4 – muscle channel

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Dt NaV1.4

Plasmid

#200250

Purpose

Expression in mammalian cells or oocytes

Depositor

Dan Minor

Article

Abderemane-Ali et al J Gen Physiol. 2021 Sep 6;153(9):e202112

Insert

Dendrobates tinctorius NaV1.4 – muscle channel

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pJH4562

Plasmid

#200780

Purpose

Pflp-18 LoxP EBFP LoxP GtACR2 mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of GtACR2 RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GtAR2

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Pflp-18

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJH4609

Plasmid

#200781

Purpose

Ppdf-1 LoxP EBFP LoxP GtACR2 mCherry unc-54 3' UTR C.elegans AVB and other neurons expression of GtACR2 RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

GtACR2

Use

Tags

mCherry

Expression

Worm

Mutation

Promoter

Ppdf-1

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJH4677

Plasmid

#200782

Purpose

Pnpr-4 ins-22::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of ins-22 GFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

ins-22 (ins-22 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

Pnpr-4

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH4783

Plasmid

#200785

Purpose

Pnpr-4 snb-1::pHluorin unc-54 3' UTR C.elegans AVA and other neurons expression of snb pHluorin

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

snb-1 (snb-1 Nematode)

Use

Tags

pHiuorin

Expression

Worm

Mutation

Promoter

Pnpr-4

Available since

May 7, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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