We narrowed to 7,125 results for: trac

1. pcDNA-NES-KIRIN1

   Plasmid

   #107416

   Purpose

   Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequence

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1
   

   Use

   Tags

   Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2MLS-HyperNova/pcDNA3

   Plasmid

   #221457

   Purpose

   Expressing HyperNova in mitochondria

   Depositor

   Kiwamu Takemoto

   Article

   Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

   Insert

   HyperNova fused with a tandem repeat of the mitochondria localization signal from cytochrome c oxidase subunit VIII
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FRB_SCF2_LD6_CTEV

   Plasmid

   #58879

   Purpose

   MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV protease

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA split protease chain with FRB rapamycin-binding ectodomain and C terminal fragment of TEV protease
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. FKBP_SCF2_LD6_NTEV

   Plasmid

   #58878

   Purpose

   MESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV protease

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA split protease chain with FKBP rapamycin-binding ectodomain and N terminal fragment of TEV protease
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. Sth1Cas9n cytosine base editor

   Plasmid

   #194209

   Purpose

   Sth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recording

   Depositor

   Chase Beisel

   Article

   Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

   Insert

   Sth1Cas9-D9A nickase
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Changed Aspartate 9 to Alanine.

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

   Plasmid

   #112927

   Purpose

   CRISPR-a reporter assay lentiviral vector (gTetO)

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. NbV5-HALOTAG

   Plasmid

   #201482

   Purpose

   Indirect detection of V5-tagged protein using the HaloTag protein labeling technology.

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   NbV5-HALOTAG
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. mNeonGreen-DEVD-NanoLuc

   Plasmid

   #98287

   Purpose

   caspase-3 BRET Sensor

   Depositor

   Maarten Merkx

   Article

   den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

   Insert

   mNeonGreen-LEHD-NanoLuc
   

   Use

   Tags

   His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRISPR-SP-Cas9 reporter

   Plasmid

   #62733

   Purpose

   Mammalian CRISPR-SP-Cas9 reporter

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   CRISPR-SP-Cas9 target seq + tdTomato (out of frame)
   

   Use

   CRISPR and Lentiviral

   Tags

   CRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-Alpha

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBR322-Pdp1_NTD-GFP-HiBiT

    Plasmid

    #198317

    Purpose

    Loads HiBiT-tagged GFP into PVCs using Pdp1 packaging domain; also contains Pnf

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1_NTD-GFP-HiBiT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBluescriptSKII+ U6-sgRNA(F+E) empty

    Plasmid

    #74707

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. 2xMyc-tdMCP-SNAP (tdMCP standard)

    Plasmid

    #226166

    Purpose

    For transient expression of the tdMCP-protein standard. Used in LABEL-seq abundance assays.

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Insert

    2xMyc-tdMCP-SNAPtag
    

    Use

    Lentiviral

    Tags

    2xMyc-tdMCP and EGFP-IRES-BlastR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP Tensin1

    Plasmid

    #105297

    Purpose

    To study localization and function of Tensin1

    Depositor

    David Critchley, Kenneth Yamada

    Article

    Clark et al J Cell Biochem. 2010 Mar 1;109(4):808-17

    Insert

    Tensin1 (TNS1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFPC1-6XHis-FLKSRP

    Plasmid

    #23001

    Depositor

    Douglas Black

    Article

    Hall et al Mol Biol Cell. 2004 Feb . 15(2):774-86.

    Insert

    KSRP (KHSRP Human)
    

    Use

    Tags

    EGFP and His

    Expression

    Mammalian

    Mutation

    valine inserted at aa#2

    Promoter

    Available since

    Nov. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.HaloTag

    Plasmid

    #209729

    Purpose

    Expresses presynaptic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (synapto).iATPSnFR2.A95A.A119L.HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. LT2_12_GlyRNA

    Plasmid

    #177710

    Purpose

    Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Inserts

    pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
    pTEFmut8-mCherry-tADH1
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. LT2_7_OxPro

    Plasmid

    #177709

    Purpose

    Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Inserts

    pTRX2_5xUAS-ymYPET-tCYC1
    pTEFmut8-mCherry-tADH1
    

    Use

    Used as donor dna in genome integration after lin…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP Tubulin K40Q

    Plasmid

    #105302

    Purpose

    To study effects of acetyl-mimetic (hyper-acetylated) tubulin

    Depositor

    Kenneth Yamada

    Article

    Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

    Insert

    alpha tubulin (TUBA1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K40Q

    Promoter

    Available since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX208_CMV::mCherry-myc-VAMP2

    Plasmid

    #199716

    Purpose

    Expresses VAMP2, fused to mCherry, in mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    mCherry-myc-VAMP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX

    Plasmid

    #167583

    Purpose

    Express the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cells

    Depositor

    Yulong Li

    Article

    Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

    Insert

    ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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