164,577 results

1. pX330A-nBRD4/PITCh

   Plasmid

   #91794

   Purpose

   PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken β-actin promoter

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNeg-Ma-barnase-TAG3-TAG45

   Plasmid

   #197572

   Purpose

   Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   Barnase - 2xTAG
   M. alvus Pyl-tRNA (6)
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   3TAG and 45TAG

   Promoter

   araC and lpp

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. SlugCas9

   Plasmid

   #163793

   Purpose

   Expresses SlugCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR- flag-Lag16-JAM-B

   Plasmid

   #205200

   Purpose

   This vector encodes of the synCAM (JAM-B) and GFP nanobody LAG16

   Depositor

   Wendell Lim

   Article

   Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

   Insert

   synCAM JAM-B and Lag16 anti-GFP nanobody
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF2250

   Plasmid

   #141991

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TCF19 (TCF19 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRES2-EGFP-p53 WT

   Plasmid

   #49242

   Purpose

   expresses human wt p53 and EGFP in mammalian cells

   Depositor

   Dylan Taatjes

   Article

   Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7-7 asyn A53T

   Plasmid

   #105727

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

   Insert

   alpha-synuclein A53T (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A53T

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. GZnP3 in pcDNA3.1+

   Plasmid

   #161738

   Purpose

   Cytosolic fluorescent reporter for Zn²⁺ (Kd = 1.3 nM, ex/em = 488 nm/515 nm)

   Depositor

   Yan Qin

   Article

   Minckley et al Nat Commun. 2019 Oct 22;10(1):4806. doi:

   Insert

   GZnP3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMPRA1

   Plasmid

   #49349

   Purpose

   Basic backbone for transient transfection of Massively Parallel Reporter Assay (MPRA) libraries

   Depositor

   Tarjei Mikkelsen

   Article

   Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. hACE2

    Plasmid

    #1786

    Purpose

    Mammalian expression of human ACE2

    Depositor

    Hyeryun Choe

    Article

    Li et al Nature. 2003 Nov 27. 426(6965):450-4.

    Has Service

    Cloning Grade DNA

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 14, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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