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pBABE-hygro-2xMyc-Rac1-P29S

Plasmid

#128581

Purpose

For mammalian cell expression of P29S mutant murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Mutation

Changed Proline29 to Serine.

Promoter

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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NLS-PyronicSF/pCMV-myc-nuc

Plasmid

#124814

Purpose

Highly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.

Depositor

Luis Felipe Barros

Article

Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

Insert

PyronicSF

Use

Tags

Nuclear Localization Signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK2-R67S/S153D

Plasmid

#213597

Purpose

Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK1 (MAPK1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R67S, S153D

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCLXSN(GFP)-myc-BioID-hp100

Plasmid

#174738

Purpose

Gene overexpression

Depositor

Shao-Cong Sun

Article

Li et al Cell Res. 2019 Jun;29(6):474-485. doi: 1

Insert

P100 (NFKB2 Human)

Use

Retroviral

Tags

Expression

Mutation

WT

Promoter

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCAMBIA-FLS2prom::FLS2-3xmyc-GFP

Plasmid

#86157

Purpose

FLS2-GFP expresssion

Depositor

Silke Robatzek

Article

Robatzek et al Genes Dev. 2006 Mar 1;20(5):537-42.

Insert

FLS2 (FLS2 Mustard Weed)

Use

Tags

3xmyc and GFP

Expression

Plant

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-Puro_hCOL4A2-Myc-His-WPRE

Plasmid

#198080

Purpose

Express hCOL4A2 in mammalian cells

Depositor

Jeffrey Miner

Insert

COL4A2 (COL4A2 Human)

Use

Tags

TEV-Myc-His

Expression

Mammalian

Mutation

Promoter

CMV, beta-globin/IgG chimeric intron

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-his-BACH1 K52R

Plasmid

#17643

Depositor

Ronny Drapkin

Article

Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

Insert

BACH1 (BRIP1 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

The lysine residue at position 52 was changed to …

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only
pPuro3.1(+)_Strep-Lrrk2(G2019S)-Myc

Plasmid

#161585

Purpose

expression of Myc-tagged mouse Lrrk2 containing Parkinson's associated G2019S mutation

Depositor

Maik Hintze

Insert

Lrrk2 (Lrrk2 Mouse)

Use

Tags

Myc tag and StrepII

Expression

Mammalian

Mutation

G2019S

Promoter

CMV

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pclbw-opa1(isoform 7DeltaS1)-myc

Plasmid

#62846

Purpose

Mammalian Expression of myc-tagged Opa1 isoform 7 Delta S1

Depositor

David Chan

Article

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mmOPA1isoform7 DeltaS1 - myc (Opa1 Mouse)

Use

Retroviral

Tags

9xMyc

Expression

Mammalian

Mutation

Promoter

cmv-bactin

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE puro mouse IRS-2 myc

Plasmid

#11373

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-2 (Irs2 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti c-MYC-DDK-Puro GFP

Plasmid

#123299

Purpose

Mammalian expression of GFP C-MYC-FLAG

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

GFP

Use

Lentiviral

Tags

MYC-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-myc-his-BACH1 P47A

Plasmid

#17644

Depositor

Ronny Drapkin

Article

Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

Insert

BACH1 (BRIP1 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

To generate the tumor-associated mutant, the prol…

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only
pLINK cyclin T1 (1-280) myc (P#586)

Plasmid

#14612

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-280) (CCNT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pPuro3.1(+)_Strep-Lrrk2(D1994S)-Myc

Plasmid

#161584

Purpose

expression of Myc-tagged kinase-dead mouse Lrrk2 containing D1994S mutation

Depositor

Maik Hintze

Insert

Lrrk2 (Lrrk2 Mouse)

Use

Tags

Myc tag and StrepII

Expression

Mammalian

Mutation

D1994S

Promoter

CMV

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-HLA-c-myc-optiT1R3 ECD

Plasmid

#113960

Purpose

mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R3 (TAS1R3 Human)

Use

Tags

Signal/leader sequence from HLA class I histocomp…

Expression

Mammalian

Mutation

residues 21-563

Promoter

cmv

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pRcCMV myc-Plk1 wt (Nigg RG6)

Plasmid

#41160

Depositor

Erich Nigg

Article

Golsteyn et al J Cell Sci. 1994 Jun;107 ( Pt 6):1509-17

Insert

Plk1 (PLK1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-Myc-GRM1-IRES-CyRFP1

Plasmid

#195355

Purpose

mGluR1-IRES-cyRFP1 under the control of the pCAG promoter

Depositor

Brenda Bloodgood

Article

Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

Insert

GRM1-IRES-CyRFP1

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fSNAP-Phleomycin

Plasmid

#188915

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-Phleomycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Myc3-Cul3 delta N41

Plasmid

#21590

Depositor

Yue Xiong

Article

Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Insert

cullin 3 (CUL3 Human)

Use

Tags

myc3

Expression

Mammalian

Mutation

Residues 34-74 deleted from N terminus.

Promoter

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-myc-ERK2-L4A-MEK1_fusion

Plasmid

#39197

Depositor

Melanie Cobb

Article

Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

Insert

ERK2-MEK1 Fusion (MAP2K1, Mapk1 Rat, Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

L4A = L33A, L37A, L40A, L42A

Promoter

CMV

Available since

July 25, 2013

Availability

Academic Institutions and Nonprofits only

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