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174,543 results

Showing: 8671 - 8680 of 174543 results

  1. lentiCRISPR_DKO

    Plasmid
    #183192
    Purpose
    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen
    Depositor
    Lang Li
    Article
    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6, mU6
    Available Since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    183192-plasmid-map-sequence-id-360098

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  2. p5154

    Plasmid
    #175390
    Purpose
    High Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)
    Depositor
    Michael Wiles
    Article
    Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10
    Type
    Empty backbone
    Use
    Mouse Targeting; Cloning vector, to be used to de…
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    175390-plasmid-map-sequence-id-348371

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  4. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

    Plasmid
    #174665
    Purpose
    Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter
    Depositor
    Eli Bar
    Inserts
    LUC2
    mCherry:T2A:Hygro
    Use
    Lentiviral and Luciferase
    Tags
    mCherry and hygromycin dual reporter gene linked …
    Expression
    Mammalian
    Promoter
    Human cytomegalovirus immediate early enhancer/pr…
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174665-plasmid-map-sequence-id-344886

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  5. Epac-S-H187

    Plasmid
    #170348
    Purpose
    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Article
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Insert
    mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available Since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    170348-plasmid-map-sequence-id-336137

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  6. XLone-Puro eGFP

    Plasmid
    #140027
    Purpose
    Tunable and temporal expression control of nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140027-plasmid-map-sequence-id-275840

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Showing: 8671 - 8680 of 174543 results