159,352 results

1. pGP-AAV-CAG-iGABASnFR2-WPRE

   Plasmid

   #218883

   Purpose

   AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Insert

   iGABASnFR2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S99A F102Y F104Y L178S

   Promoter

   CAG

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. px330_Rosa_sgRNA

   Plasmid

   #97007

   Purpose

   Expresses Cas9 and Rosa26 locus specific sgRNA

   Depositor

   Russell Ray

   Article

   Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

   Insert

   Rosa26 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-mTagBFP2-T2A-FLPo

   Plasmid

   #124834

   Purpose

   Monomeric TagBFP2 and FLP recombinase

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-FLPo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. EDNRA-DuET

   Plasmid

   #213232

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   EDNRA (EDNRA Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKGmDUET-NgBR/hCIT

   Plasmid

   #203173

   Purpose

   Expression of human cis-prenyltransferase complex for purification

   Depositor

   William Sessa

   Article

   Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

   Inserts

   NUS1 (NUS1 Human)
   DHDDS (DHDDS Human)
   

   Use

   Tags

   Strep-Tag II-TEV and internally 6HIS tagged

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Synapsin-pm-iATPSnFR1.0

   Plasmid

   #102555

   Purpose

   expresses membrane displayed ATP sensor in neurons

   Depositor

   Baljit Khakh

   Article

   Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

   Insert

   iATPSnFR1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOz-humBirA-GFP

   Plasmid

   #133283

   Purpose

   Expression of BirA-GFP fusions in mammalian cells

   Depositor

   Arman Kulyyassov

   Article

   Kulyyassov et al Biomolecules. 2020 Jan 16;10(1). pii: bi

   Insert

   eGFP
   

   Use

   Retroviral

   Tags

   Humanized wild type biotin ligase BirA

   Expression

   Mammalian

   Mutation

   Promoter

   MoMuLV

   Available since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD02_CBASS_Escherichia_coli_SLK172

   Plasmid

   #224395

   Purpose

   For expression of the type I CBASS system encoded by Escherichia coli SLK172

   Depositor

   Philip Kranzusch

   Article

   Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

   Insert

   EcCdnD_4TM
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. paavCAG-pre-mGRASP-mCerulean

   Plasmid

   #34910

   Purpose

   pre

   Depositor

   Jinhyun Kim

   Article

   Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

   Insert

   presynaptic mGRASP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Nov. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBiT3.1-N_STRAP:1-350

    Plasmid

    #211139

    Purpose

    Mammalian expression of full-length human STRAP. N-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    STRAP:M1-A350 (STRAP Human)
    

    Use

    Tags

    MVSGWRLFKKISGSSGGSSG

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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