We narrowed to 2,543 results for: LRE
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Plasmid#72797PurposeC-terminal tagging with mCherry2 using CRIPSR/Cas9DepositorInsertmCherry2-Hygro
UseCRISPRTagsmCherry2ExpressionMammalianAvailable SinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Dyskerin
Plasmid#207577PurposeN-terminal mCherry-tag using SalI/EcoRIDepositorInsertgenerated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
TagsmCherryExpressionMammalianPromoterLTRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70
Plasmid#62951PurposeCreating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP.DepositorInsert3XGal80-FLAG/HA/EGFP-Hsp70
Available SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
MC177VSG221RNAi
Plasmid#59730PurposeFor use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.DepositorInsertVSG221 RNAi fragment
UseRNAiPromoteropposing T7 promotersAvailable SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
R26-EGFP HMEJ donor vector
Plasmid#137927PurposeROSA26 knock-in vector with homology-mediated end joining(HMEJ)-based methodDepositorInsertSA-EGFP-bpA with homology arms
UseMouse TargetingAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Cdk9 T186A
Plasmid#28101DepositorInsertCdk9 (CDK9 Human)
TagsFLAGExpressionBacterial and MammalianMutationContains a T186A mutation in Cdk9Available SinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-Halo-Dyskerin
Plasmid#207578PurposeCRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locusDepositorInsertHaloTag DKC1
TagsHaloTagExpressionMammalianPromoterCMVAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCX-SpiCee-NES
Plasmid#140901Purposemammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signalingDepositorInsertSpiCee-NES
TagsmRFPExpressionMammalianPromoterCAGAvailable SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgSlc18a2
Plasmid#124849PurposeMutagenesis of Slc18a2DepositorInsertSlc18a2 (Slc18a2 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -