We narrowed to 23,622 results for: CHI

1. pKLV2-U6gRNA5(NR5A2_5-2)-PGKpuroBFP-W

   Plasmid

   #211976

   Purpose

   Express gRNA against NR5A2 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting NR5A2 (NR5A2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKLV2-U6gRNA5(NR5A2_5-5)-PGKpuroBFP-W

   Plasmid

   #211977

   Purpose

   Express gRNA against NR5A2 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting NR5A2 (NR5A2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKLV2-U6gRNA5(PDLIM1_5-1)-PGKpuroBFP-W

   Plasmid

   #211978

   Purpose

   Express gRNA against PDLIM1 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting PDLIM1 (PDLIM1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKLV2-U6gRNA5(PDLIM1_Bru)-PGKpuroBFP-W

   Plasmid

   #211979

   Purpose

   Express gRNA against PDLIM1 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting PDLIM1 (PDLIM1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKLV2-U6gRNA5(MYCN_5-3)-PGKpuroBFP-W

   Plasmid

   #211972

   Purpose

   Express gRNA against MYCN with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting MYCN (MYCN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKLV2-U6gRNA5(MYCN_5-4)-PGKpuroBFP-W

   Plasmid

   #211973

   Purpose

   Express gRNA against MYCN with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting MYCN (MYCN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPV-C1-crRNA(DMD#20_DMD#23)-EF1a-BA

   Plasmid

   #204620

   Purpose

   Expression vector of crRNA (DMD#20) targeting the dystrophin intron 44 just upstream of exon 45, and crRNA(DMD#23)targeting the dystrophin intron 55 just downstream of exon 55. Blasticidin selectable.

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Insert

   crRNA#20 (targeting DMD intron44) and crRNA#23 (targeting DMD intron 55)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-Sox2AV

   Plasmid

   #193354

   Purpose

   Retroviral vector expressing mouse Sox2A61V point mutant

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Sox2AV (Sox2 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Sox2A61V is a highly cooperative point mutant of …

   Promoter

   5'LTR

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-hSOX2AV

   Plasmid

   #193352

   Purpose

   Retroviral vector expressing human SOX2A61V point mutant

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   SOX2AV (SOX2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   SOX2A61V is a highly cooperative point mutant of …

   Promoter

   5'LTR

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSico_U6-PLOD2 sgRNA3

    Plasmid

    #136458

    Purpose

    Expression of gRNA against human PLOD2

    Depositor

    Antonio Amelio

    Article

    Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

    Insert

    gRNA against human PLOD2 (PLOD2 Synthetic, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSico_U6-PLOD2 sgRNA5

    Plasmid

    #136460

    Purpose

    Expression of gRNA against human PLOD2

    Depositor

    Antonio Amelio

    Article

    Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

    Insert

    gRNA against human PLOD2 (PLOD2 Synthetic, Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. px335-sgRNA-EF(Cas9n)-mHdac1-L2

    Plasmid

    #122307

    Purpose

    Expresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Hdac1 (Hdac1 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc-GFP-dSUMOdeltaC

    Plasmid

    #85697

    Purpose

    expression of GFP-SUMO1 delta C in Drosophila cells

    Depositor

    Giannino Del Sal, Licio Collavin

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    SUMO (Sumo Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    non-conjugatable SUMO, lacking the C-terminal gly…

    Promoter

    pAc5

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pUCmini-iCAP-AAV.MaCPNS2

    Plasmid

    #185137

    Purpose

    non-standard AAV2 rep-AAV-MaCPNS2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

    Insert

    Synthetic construct isolate AAV-MaCPNS2 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUCmini-iCAP-AAV.MaCPNS1

    Plasmid

    #185136

    Purpose

    non-standard AAV2 rep-AAV-MaCPNS1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

    Insert

    Synthetic construct isolate AAV-MaCPNS1 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV

    Plasmid

    #216164

    Purpose

    Expression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    Gaussia princeps luciferase (with double mutation to improve stability)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-BACE1-mBFP

    Plasmid

    #196697

    Purpose

    Expresses Bace1 with an HA tag at N-terminal for single molecule tracking and other measusers in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Beta-secretase 1 (BACE1 Human)
    

    Use

    Tags

    HA and mBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-APP-mGFP

    Plasmid

    #196696

    Purpose

    Expresses APP695 with an HA tag an N-terminal for single molecule tracking and other measusers in mammalian cells

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    HA and mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCSIIpuro-EGF-ScNeo

    Plasmid

    #209893

    Purpose

    To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    EGF-ScNeo (EGF Human)
    

    Use

    Lentiviral

    Tags

    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCSIIpuro-HBEGF-ScNeo

    Plasmid

    #209894

    Purpose

    To monitor the status of HB-EGF, the plasmid encodes a recombinant HB-EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    HBEGF-ScNeo (HBEGF Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1-IDO1-p2a-eGFP

    Plasmid

    #187026

    Purpose

    Expresses IDO1 and GFP (seperately) in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

    Insert

    Indoleamine 2,3-dioxygenase 1 (IDO1 Human)
    

    Use

    Tags

    P2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. TDP-REGv1(mCherry-FLAG) in pTwist-CMV

    Plasmid

    #216165

    Purpose

    Expression of mCherry specifically in cells with TDP-43 knockdown. Uses AARS1-derived cryptic exon to control mCherry expression. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mCherry with C-terminal FLAG tag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHLS-EF1a-FRB-SpCas9-A

    Plasmid

    #138477

    Purpose

    Expresses FRB fused SpCas9 for NanoMEDIC packaging.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    SpCas9, human codon optimized
    

    Use

    CRISPR

    Tags

    3x HA tag and FRB

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMXS-IRES-Blast mAtg7

    Plasmid

    #173170

    Purpose

    Retroviral vector to express sgRNA resistant mouse Atg7

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Cell Metab. 2020 Oct 28. pii: S1550-4131

    Insert

    Autophagy Related 7 (Atg7 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available since

    Feb. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPBbsr2-AREG-ScNeo

    Plasmid

    #209910

    Purpose

    To monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    AREG-ScNeo (AREG Human)
    

    Use

    Tags

    mNeonGreen and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPBbleo-EGF-ScNeo

    Plasmid

    #209902

    Purpose

    To monitor the status of EGF, the plasmid encodes a recombinant EGF fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    EGF-ScNeo (EGF )
    

    Use

    Tags

    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCSIIpuro-AREG-ScNeo

    Plasmid

    #209897

    Purpose

    To monitor the status of Amphiregulin, the plasmid encodes a recombinant AREG fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    AREG-ScNeo (AREG Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TDP-REGv2 Cryptic PE-Max

    Plasmid

    #216163

    Purpose

    Expression of prime editor PEMax (Plasmid #174820) only in cells with TDP-43 loss of function. Note: It is not recommended to produce lentivirus containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    PEMax for prime editing with internal cryptic exon
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCSIIpuro-EPGN-ScNeo

    Plasmid

    #209899

    Purpose

    To monitor the status of Epigen, the plasmid encodes a recombinant Epigen fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    EPGN-ScNeo (EPGN Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCSIIpuro-EREG-ScNeo

    Plasmid

    #209896

    Purpose

    To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    EREG-ScNeo (EREG Human)
    

    Use

    Lentiviral

    Tags

    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge