We narrowed to 13,583 results for: TIM

1. LT2_7_OxPro

   Plasmid

   #177709

   Purpose

   Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Inserts

   pTRX2_5xUAS-ymYPET-tCYC1
   pTEFmut8-mCherry-tADH1
   

   Use

   Used as donor dna in genome integration after lin…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-b2AR-eLOV-TEVcs-FLAG-GAL4-V5

   Plasmid

   #104841

   Purpose

   b2AR-SPARK TF component

   Depositor

   Alice Ting

   Article

   Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

   Insert

   b2AR-eLOV-TEVcs-FLAG-GAL4-V5
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. tetO-TBX5

   Plasmid

   #46032

   Depositor

   John Gearhart

   Article

   Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

   Insert

   TBX5 (TBX5 Human)
   

   Use

   Lentiviral

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. 12URA-B-ScTop2∆CTD

   Plasmid

   #214924

   Purpose

   Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

   Depositor

   James Berger

   Article

   Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

   Insert

   topoisomerase II
   

   Use

   Tags

   6x His-TEV

   Expression

   Yeast

   Mutation

   Deleted amino acids 1178-1428

   Promoter

   Gal 1/10

   Available since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLNCX2-mCherry-VPS4A

   Plasmid

   #115334

   Purpose

   expresses mCherry-VPS4A in mammalian cells

   Depositor

   Sanford Simon

   Article

   Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

   Insert

   VPS4A (VPS4A Human)
   

   Use

   Retroviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   silent mutation in position 4050 C to T

   Promoter

   CMV

   Available since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDA_112

   Plasmid

   #136475

   Purpose

   Lentiviral expression of AsCas12a

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   AsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBBRBB-eGFP

   Plasmid

   #32549

   Depositor

   Claudia Schmidt-dannert

   Article

   Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

   Insert

   eGFP (eGFP )
   

   Use

   Synthetic Biology

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Consitutive lac

   Available since

   Nov. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

   Plasmid

   #63168

   Purpose

   GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-V5"
   

   Use

   Tags

   N-myristoylation

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3NES-CS(G)-tTA-CS(G)-3NES

   Plasmid

   #137836

   Purpose

   Soluble tTA transcription factor flanked by N and C terminal TEV protease G cleavage sequences and 3 nuclear export sequences

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   3NES-CD(G)-tTA-CS(G)-3NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMito-Electra2

    Plasmid

    #184927

    Purpose

    Mitochondrial labeling with blue fluorescent protein Electra2 fused to partial COX8A

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    COX8A-Electra2
    

    Use

    Tags

    Electra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. LT3REVIR

    Plasmid

    #111176

    Purpose

    Tet-ON miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOS014E: Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)

    Plasmid

    #163072

    Purpose

    Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    Grx1-roGFP2
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-ChR2-mRuby2-ST

    Plasmid

    #109125

    Purpose

    Cation channelrhodopsin ChR2 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChR2-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Syn-ChR2(H134R)-GFP

    Plasmid

    #58880

    Purpose

    AAV expression of ChR2(H134R)-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

    Has Service

    AAV Retrograde and AAV8

    Insert

    ChR2(H134R)-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    Syn

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAJM.336

    Plasmid

    #108528

    Purpose

    LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    LacIAM + PTac-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LacI Q18M A47V F161Y

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA_DENV2-NS2B3_V5

    Plasmid

    #115906

    Purpose

    Mammalian expression of DENV2 NS2B3 protein with a C-terminal V5 tag

    Depositor

    Alan Rothman

    Article

    Udawatte et al Front Cell Infect Microbiol. 2022 Oct 14

    Insert

    DENV2 NS2B3 protease
    

    Use

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. flex-ChrimsonR-EYFP-Kv

    Plasmid

    #135319

    Purpose

    Cre-dependent expression of soma-targeted ChrimsonR

    Depositor

    Hongkui Zeng

    Article

    Hage et al Elife. 2022 Jan 21;11:e71103. doi: 10.75

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    synapsin

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. TagRFP-T-VAPB(1- 218)- eMagB

    Plasmid

    #162255

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    TagRFP-T-VAPB(1- 218)- eMagB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    beta-actin promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. PKG I alpha WT

    Plasmid

    #16390

    Depositor

    Bernard Weinstein

    Article

    Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

    Insert

    PKG1 alpha (PRKG1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1/hChR2(H134R)-EYFP

    Plasmid

    #20940

    Purpose

    Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    CMV

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAJM.847

    Plasmid

    #108524

    Purpose

    PhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PhlFAM + PPhlF-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    PhlF: S50G S73I R95H A121V M137I N144D T155I R200C

    Promoter

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. hum sgk promoter F4R4

    Plasmid

    #83426

    Purpose

    promoter reporter construct to test 5' flanking sequences

    Depositor

    Christie Thomas

    Article

    Itani et al Am J Physiol Endocrinol Metab. 2002 Nov;

    Insert

    human Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    sgk

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mNG-sfTq2ox

    Plasmid

    #124219

    Purpose

    Cytoplasmic mNeongreen superfolder mTurquoise2 ox tandem, ~60 % energy transfer. pNM045 - pSAV057-mNG-sfTq2ox

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    mNG-sfTq2ox
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRDA_888

    Plasmid

    #201165

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCFD2-dU6:2gRNA

    Plasmid

    #49409

    Purpose

    expression of gRNA under control of the Drosophila U6:2 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-2:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-2

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EC64422

    Plasmid

    #191768

    Purpose

    CRISPR mutagenesis in Medicago truncatula

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:221-252. doi

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pENO1-dTom-NATr

    Plasmid

    #52208

    Purpose

    Constitutive expression of Candida codon-optimized dTomato with NAT selectable marker

    Depositor

    Robert Wheeler

    Article

    Gratacap et al Dis Model Mech. 2013 Sep;6(5):1260-70. d

    Insert

    dTomato
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for Candida species

    Promoter

    ENO1

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

28. Plasmid L5 attB::Pleft* mCherry

    Plasmid

    #169411

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for mCherry

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    pLeft
    mCherry
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Plasmid L5 attB:Psmyc double blue

    Plasmid

    #170050

    Purpose

    Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double blue

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mTagBFP2
    CO BFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAJM.661

    Plasmid

    #108532

    Purpose

    TtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    TtgRAM + PTtg-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    TtgR: R130H A144T A148V

    Promoter

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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