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We narrowed to 15,323 results for: pET

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Showing: 3441 - 3480 of 15323 results

  1. pET28-Flag-RBM24-95~236

    Plasmid
    #208634
    Purpose
    for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa95~236
    Depositor
    Quanfu Ma
    Insert
    human RBM24 (ENST00000216146) aa 95~236 (RBM24 Human)
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    only aa 95~236, other residues not included
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208634-plasmid-map-sequence-id-418349

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  2. pET28-mEGFP(A206K)-TwStrep

    Plasmid
    #208622
    Purpose
    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone
    Depositor
    Quanfu Ma
    Insert
    mEGFP(A206K)
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    (compared with pEGFP-C1, protein level) A206K
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208622-plasmid-map-sequence-id-415916

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  6. pETM11-6xHis-TEV-hORF1p_StammerAAA

    Plasmid
    #202587
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202587-plasmid-map-sequence-id-415633

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  7. pETM11-6xHis-TEV-hORF1p_StammerAEA

    Plasmid
    #202588
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Methionine 91 to Alanine, changed O…
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202588-plasmid-map-sequence-id-415632

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  33. pETM-60-NdeI-NcQde-2_V

    Plasmid
    #147747
    Purpose
    Bacterial Expression of NcQde-2
    Depositor
    Elisa Izaurralde
    Insert
    NcQde-2
    Expression
    Bacterial
    Mutation
    1 non silent mutation P761S compared to the seque…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147747-plasmid-map-sequence-id-290194

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  38. pET15b I-SceI (Ctermhis) K223I

    Plasmid
    #195615
    Purpose
    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity
    Depositor
    Frederick Gimble
    Article
    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.
    Insert
    I-SceI
    Expression
    Bacterial
    Mutation
    C2694G, G2345C, G2351C, A3365T, A3366T
    Promoter
    T7
    Available Since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195615-plasmid-map-sequence-id-383680

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  39. pET15b I-SceI (Ctermhis) K122I

    Plasmid
    #195614
    Purpose
    Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activity
    Depositor
    Frederick Gimble
    Article
    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.
    Insert
    I-SceI
    Expression
    Bacterial
    Mutation
    C2458G, A2826T, A2827T
    Promoter
    T7
    Available Since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195614-plasmid-map-sequence-id-383676

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Showing: 3441 - 3480 of 15323 results