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pJK220

Plasmid

#71123

Purpose

Expression of E. coli pantotheinate kinase (H6PanK, R175H mutant) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

pantothenate kinase

Tags

H6

Expression

Bacterial

Mutation

Arg175 is mutated to a His

Promoter

T7

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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p2lox-cAMPr R626A

Plasmid

#102437

Purpose

cAMPr with a point mutation R626A which theoretically decreases its affinity for cAMP

Depositor

Justin Blau

Article

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr

Use

Cre/Lox

Expression

Mammalian

Mutation

PKA-R R626A

Available Since

Feb. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCri-4a

Plasmid

#61314

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and TRX-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pDUET-ctCPR-trAMO

Plasmid

#20112

Depositor

Jay Keasling

Article

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

p450 reductase and truncated amorphadiene oxidase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCri-6a

Plasmid

#61315

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

GST-tag and His-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pCri-12b

Plasmid

#61334

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Bacterial

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pCri-9a-Strep

Plasmid

#61341

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

Strep-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pGBW-m4046465

Plasmid

#149112

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF7a protein

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 ORF7a protein (ORF7a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available Since

May 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCri-15b

Plasmid

#61336

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Yeast

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCri-1a

Plasmid

#60816

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and MBP-tag

Expression

Bacterial

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCri-16a

Plasmid

#61324

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Bacterial

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCri-14a

Plasmid

#61322

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and LSL-tag

Expression

Bacterial

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only
pCri-8a-Strep

Plasmid

#61340

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Article

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag and Strep-tag

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
tagRFP-GPHN.FingR-HaloTag

Plasmid

#236112

Purpose

Half of chemically inducible GFE3. When co-expressed with 236113 can degrade inhibitory synapses in presence of TH. (trimethoprim-HaloTag ligand).

Depositor

Don Arnold

Article

Bareghamyan et al eLife. 2025 Apr 29;13:RP103757. doi: 10.

Insert

tagRFP-GPHN.FingR-HaloTag

Expression

Mammalian

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pBSII-FRT-MCS

Plasmid

#205988

Purpose

Vector containing FRT and FRT-F3 sites

Depositor

Sharon Torigoe

Article

Waite et al PLoS One. 2024 Sep 30;19(9):e0311120. do

Type

Empty backbone

Use

Pcr vector

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_T2_gRNA

Plasmid

#199388

Purpose

pDEL112; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type II sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 type II sgRNA

Use

Tol2 destination vector

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET30a- phage MazG-like/Atd1

Plasmid

#195244

Purpose

Expersses phage MazG-like/Atd1

Depositor

Douglas Feldman

Article

Ho et al Cell Rep. 2023 Mar 22;42(4):112305. doi:

Insert

phage MazG

Tags

Flag and TwinStrep

Expression

Bacterial

Mutation

Catalytic site mutant

Promoter

T7

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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Prp39-pET21b

Plasmid

#111286

Purpose

Prp39 expression plasmid

Depositor

Christine Guthrie

Article

Price et al RNA. 2014 Jan;20(1):46-60. doi: 10.1261/

Insert

PRP39

Tags

his6

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETA-Prp28

Plasmid

#111285

Purpose

Prp28 expression plasmid

Depositor

Christine Guthrie

Article

Price et al RNA. 2014 Jan;20(1):46-60. doi: 10.1261/

Insert

PRP28

Tags

His

Expression

Bacterial

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Prp40-pET21b

Plasmid

#111287

Purpose

Prp40 expression plasmid

Depositor

Christine Guthrie

Article

Price et al RNA. 2014 Jan;20(1):46-60. doi: 10.1261/

Insert

PRP40

Tags

his6

Expression

Bacterial

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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