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pcDNA3 PARL-FLAG-CT H335G

Plasmid

#13749

Depositor

Luca Pellegrini

Article

Cipolat et al Cell. 2006 Jul 14. 126(1):163-75.

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

Changed catalytic His 335 to Gly

Available Since

Feb. 1, 2007

Availability

Academic Institutions and Nonprofits only
pHA-N1-GalT

Plasmid

#87326

Purpose

To express GalT-HA in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase

Depositor

Lei Lu

Article

Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

Insert

1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV-F

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pU6-1 (GB1204)

Plasmid

#75405

Purpose

Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

AtU6-1 promoter

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pSMP-Ehmt1_3

Plasmid

#36337

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Ehmt1 (EHMT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
His-MBP-NRBF2 (1-159)

Plasmid

#99331

Purpose

Mammalian expression of the monomeric form of NRBF2 that binds with the PI3K C1 complex

Depositor

James Hurley

Article

Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

Insert

Nuclear receptor-binding factor 2 (NRBF2 Human)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Mutation

AA1-159 are present

Promoter

T7

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-CMV-Cdc42 3'UTR mutant (position 577-599)

Plasmid

#51391

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

NM_001039802.1 (CDC42 Human)

Use

Luciferase

Tags

Luciferase

Mutation

miR-29 binding site mutant (position 577-599)

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-4T3-ZO-2-1st-PDZ-delta-NLS

Plasmid

#27425

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

GST

Expression

Bacterial

Mutation

Deletion of NLS-Nuclear Localization Signal: Nucl…

Available Since

May 18, 2011

Availability

Academic Institutions and Nonprofits only
pBABEhygro-Flag-ZO-2-delta-N

Plasmid

#27476

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Deletion of coding nucleotides from 1-351. This r…

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL4C-V5-His6

Plasmid

#67423

Purpose

Expression of hArl4c with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL4C

Plasmid

#67465

Purpose

Mammalian expression of hArl4c with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pRS406-PHO5-GFP-CERT PH domain EE mutation

Plasmid

#58833

Purpose

Expresses GFP-Tagged and mutated CERT PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Arginine 43 to Glutamate

Promoter

PHO5

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
myc-Med22 pBacPAK8

Plasmid

#15374

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

Med22 (MED22 Human)

Use

Baculovirus construction

Tags

myc

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pAcGP67A-mTLR4ecd-VLR

Plasmid

#187877

Purpose

Baculovirus protein expression of mouse TLR4 (residues 26–544) fused with hagfish variable lymphocyte receptor (VLR) (residues 126–200)

Depositor

Bruce Beutler

Article

Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

TLR4ecd (Tlr4 Mouse)

Expression

Insect

Mutation

includes residues 26–544 of mouse TLR4

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_delta_EHMT1

Plasmid

#228816

Purpose

Expression of human EHMT1 with deletion of region between N-terminal 300 aa and C-terminal Ank+SET

Depositor

Shravanti Rampalli

Article

Biligiri et al PLOS Biology 22(11): e3002871.

Insert

EHMT1 (EHMT1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of region between N-terminal 300 aa and …

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV8-3

Plasmid

#237961

Purpose

Encodes TRAV8-3 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV8-3 (TRAV8-3 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgDHFR

Plasmid

#233898

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting DHFR

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

sgRNA targeting DHFR (DHFR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE Flag HA MPG Catalytic Inactive

Plasmid

#237807

Purpose

Lentiviral vector expressing Catalytic Inactive MPG

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

MPG (MPG Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[G249D]-P2A-Electra1 (pAH10)

Plasmid

#227615

Purpose

Overexpression of SLC25A46 with G249D mutation in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

G249D

Promoter

CMV, T7

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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PE-pegRNA(BG)-nsgRNA(BG)

Plasmid

#232727

Purpose

A dual-targeting vector that contains PE3 system guide RNAs against BFP (pegRNA(BG) and nsgRNA(BG)) with restriction enzyme sites for insertion of target site guide RNAS (pegRNAs(TS) and nsgRNA(TS)).

Depositor

David Brafman

Article

Frisch et al Mol Ther Nucleic Acids. 2023 Jul 17;33:4

Insert

pegRNA(BG), nsgRNA(BG), pegRNAs(TS), nsgRNA(TS)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2D-A

Plasmid

#221713

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D683A in active site

Promoter

simian CMV IE94

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

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