We narrowed to 30,431 results for: Ide

1. pSP6-RNAP

   Plasmid

   #48143

   Purpose

   Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   rna polymerase SP6
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   V314A (numbering based on NCBI Sequence ID: NP_85…

   Available Since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTT3 Unc5C-FLAG

   Plasmid

   #72196

   Purpose

   Expresses full-length Unc5c with a C-terminal FLAG tag

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Unc5c (Unc5c Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. GST-CHMP4A (1-204) (pGEX2T(TEV))

   Plasmid

   #80632

   Purpose

   Residues 1-204 of CHMP4A; Bacterial Expression vector; TEV-cleavable GST tag; Sundquist Lab internal ID: WISP06-198

   Depositor

   Wesley Sundquist

   Article

   McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

   Insert

   CHMP4A (CHMP4A Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Residues 1-204

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ntn1-Fc-His

   Plasmid

   #72104

   Purpose

   Expresses the entire Netrin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ntn1 (Ntn1 Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Plxna1-AP-His

   Plasmid

   #71996

   Purpose

   Expresses the extracellular region of the PlexinA1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Plxna1 (Plxna1 Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEM-Cas9HF1-recA56

   Plasmid

   #89962

   Purpose

   Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Inserts

   Cas9HF1
   recA56
   

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet and proD

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Plxna1-Fc-His

   Plasmid

   #72122

   Purpose

   Expresses the extracellular region of the PlexinA1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Plxna1 (Plxna1 Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Unc5b.z-AP-His

   Plasmid

   #72053

   Purpose

   Expresses the extracellular region of the Unc5B, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Unc5b.z (Unc5b Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-BLM

   Plasmid

   #80070

   Purpose

   Expresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)

   Depositor

   Nathan Ellis

   Article

   Hu et al Hum Mol Genet. 2001 Jun 1;10(12):1287-98

   Insert

   BLM (BLM Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   If correct ID, the cDNA is from HeLa and has char…

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-OSF-KATNA1

    Plasmid

    #160051

    Purpose

    Mammalian expression vector for KATNA1 (KATANIN-P60) with N-terminal One-Strep-Flag tag. Internal ID: WISP20-18.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Tags

    One-Strep-Flag (OSF)

    Expression

    Mammalian

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTT3 Unc5B-FLAG

    Plasmid

    #72195

    Purpose

    Expresses full-length Unc5b with a C-terminal FLAG tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5b (Unc5b Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGRG36-Kn-PA1-GFP

    Plasmid

    #79088

    Purpose

    pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to integrate a GFP expression cassette into the chromosome of E.coli, described in Yang et al. 2016 (Pending publication Pubmed ID)

    Depositor

    Frank Rosenzweig

    Article

    Yang et al Appl Environ Microbiol. 2020 Apr 1;86(8)

    Insert

    GFP
    

    Use

    Bacterial chromosome integration and expression o…

    Expression

    Bacterial

    Promoter

    PA1

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBtPIPLC His

    Plasmid

    #173808

    Purpose

    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

    Depositor

    Anne Gershenson, Mary F Roberts

    Article

    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

    Insert

    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Plxna4-Fc-His

    Plasmid

    #72125

    Purpose

    Expresses the extracellular region of the PlexinA4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Plxna4 (Plxna4 Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEM-Cas9HF1-indRecA56

    Plasmid

    #102294

    Purpose

    Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Inserts

    Cas9HF1
    recA56
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    N497A/R661A/Q695A/Q926A

    Promoter

    pTet

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTT3 Unc5D-FLAG

    Plasmid

    #72197

    Purpose

    Expresses full-length Unc5d with a C-terminal FLAG tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5d (Unc5d Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Sema6a.a-AP-His

    Plasmid

    #72037

    Purpose

    Expresses the extracellular region of the Sema6A, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6a.a (Sema6a Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Sema6d.4-AP-His

    Plasmid

    #72044

    Purpose

    Expresses the extracellular region of the Sema6D, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.4 (Sema6d Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQFmcs-2H12.D11-scarREF

    Plasmid

    #221184

    Purpose

    cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen2
    mScarlet-I
    

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    None (same as cdGreen2) and PQ5 (cumate-inducible)

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Sema3c(L)-AP-His

    Plasmid

    #72015

    Purpose

    Expresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3c (Sema3c Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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