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We narrowed to 2,405 results for: dcas9

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Showing: 1721 - 1740 of 2405 results

  3. pEHE51

    Plasmid
    #250522
    Purpose
    Vector for sgRNA expression in Acinetobacter baumannii (CRISPRi). Modified version of pYDE007 (Plasmid #194152) made Golden Gate compatible (BsaI) for sgRNA oligo insertion.
    Depositor
    Ralph Isberg
    Publication
    Hamami et al bioRxiv [Preprint]. 2025 Aug 4:2024.01.0
    Insert
    Two BsaI sites (T-BsaI-BfuI-BsaI-A) inserted between the J23119 promoter and dCas9 handle/gRNA scaffold
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    250522-plasmid-map-sequence-id-497877

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  9. KL401: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-Dnmt3a3L

    Plasmid
    #121840
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS/Dnmt3a-3L scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121840-plasmid-map-sequence-id-237394

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  13. sgRNA_expression_vector

    Plasmid
    #210212
    Purpose
    An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.
    Depositor
    Albert Jeltsch
    Publication
    Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi
    Type
    Empty backbone
    Expression
    Bacterial and Mammalian
    Promoter
    U6
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    210212-plasmid-map-sequence-id-416400

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  16. JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold

    Plasmid
    #121841
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121841-plasmid-map-sequence-id-237395

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Showing: 1721 - 1740 of 2405 results