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175,069 results

Showing: 8741 - 8750 of 175069 results

  4. pBZCas13b

    Plasmid
    #89898
    Purpose
    Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.
    Depositor
    Feng Zhang
    Article
    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.
    Insert
    Cas13b
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89898-plasmid-map-sequence-id-178222

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  7. AICSDP-23:TJP1-mEGFP

    Plasmid
    #87429
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    TJP1 Homology Arms with mEGFP-linker (TJP1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    March 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87429-plasmid-map-sequence-id-174170

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  8. Hygro-Cas9 donor

    Plasmid
    #86883
    Purpose
    Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selection
    Depositor
    Danwei Huangfu
    Article
    Shi et al Cell Stem Cell. 2017 Feb 8. pii: S1934-5
    Insert
    hSpCas9
    Use
    CRISPR
    Tags
    3xFlag, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Available Since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86883-plasmid-map-sequence-id-173280

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  10. pDule-tfmF A65V S158A

    Plasmid
    #85484
    Purpose
    Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA
    Depositor
    Ryan Mehl
    Article
    Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.
    Insert
    tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
    Tags
    None
    Expression
    Bacterial
    Mutation
    Y32V F108W Q109M I159M with 65Val and 158Ala
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85484-plasmid-map-sequence-id-169864

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Showing: 8741 - 8750 of 175069 results